Anti-Mouse CD25 – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Mouse CD25 – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: C526

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Clone
PC61
Target
CD25
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IL2RA, IDDM10, CD25, IL2R, TCGFR, P55, Tac
Isotype
IgG1 λ
Applications
IHC FF
,
PhenoCycler®

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Product Size
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CODEX® Details

Validation Notes
Clone PC61 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue.
Tissue Screened
Spleen
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse
Host Species
Rat
Concentration
0.5 mg/ml
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD25 (Clone PC61) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2892849
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone PC61 recognizes an epitope on mouse CD25.
Antigen Distribution
CD25 is expressed in activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells.
Background
CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25.

Antigen Details

Protein
Ligand/Receptor
IL-2
PubMed
NCBI Gene Bank ID

References & Citations

1. Warren J. Leonard et al. (2002) The EMBO Journal 21: 3051
2. FW Alt et al. (1995) Immnnity 3: 521
3. Greene WC et al. (1990) J Invest Dermatol. 94: 27S
IHC
PhenoCycler®

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.