Anti-Mouse CD25 – Purified (PhenoCyler-Fusion (CODEX)® Ready)
Anti-Mouse CD25 – Purified (PhenoCyler-Fusion (CODEX)® Ready)
Product No.: C526
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Clone PC61 Target CD25 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names IL2RA, IDDM10, CD25, IL2R, TCGFR, P55, Tac Isotype IgG1 λ Applications IHC FF , PhenoCycler® |
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CODEX® DetailsValidation Notes Clone PC61 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue. Tissue Screened Spleen Tissue Preparation FF (Fresh Frozen) Antibody and Reporter DetailsReactivity Species Mouse Host Species Rat Concentration 0.5 mg/ml Immunogen IL-2-dependent cytolytic mouse T-cell clone B6.1 Formulation This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4. Storage and Handling This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Applications and Recommended Usage? Quality Tested by Leinco CODEX® This CD25 (Clone PC61) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml. RRID AB_2892849 Country of Origin USA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone PC61 recognizes an epitope on mouse CD25. Antigen Distribution CD25 is expressed in activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells. Background CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25. Antigen DetailsProtein Ligand/Receptor IL-2 PubMed NCBI Gene Bank ID References & Citations1. Warren J. Leonard et al. (2002) The EMBO Journal 21: 3051 2. FW Alt et al. (1995) Immnnity 3: 521 3. Greene WC et al. (1990) J Invest Dermatol. 94: 27S Technical Protocols PhenoCycler® |
Formats Available
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Prod No. | Description |
|---|---|
C1924 | |
C1189 | |
C1193 | |
C1192 | |
C1191 | |
C1916 | |
C1917 | |
C1918 | |
C1920 | |
C1922 | |
C526 | |
C1194 | |
C1190 | |
C2845 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
