Anti-Mouse CD25 – Purified In vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C2845
Clone
PC61
Protein
CD25
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IL2RA, IDDM10, CD25, IL2R, TCGFR, P55, Tac, IL-2 Rα
Isotype
Rat
IgG1 λ
Applications
B
,
CODEX®
,
Depletion
,
FA
,
FC
,
IHC FF
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2845-1.0 mg
1.0 mg
$198.00
In stock
Max:
Min: 1
Step: 1
C2845-5.0 mg
5.0 mg
$355.00
In stock
Max:
Min: 1
Step: 1
C2845-25 mg
25 mg
$1,025.00
In stock
Max:
Min: 1
Step: 1
C2845-50 mg
50 mg
$1,650.00
In stock
Max:
Min: 1
Step: 1
C2845-100 mg
100 mg
$2,220.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this PC61 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this PC61 antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
CODEX®
B
Depletion
IHC (Frozen)
IP
FA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone PC61 recognizes an epitope on mouse CD25.
Antigen Distribution
CD25 is expressed in activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells.
Background
CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25.

Antigen Details

Protein
Ligand/Receptor
IL-2
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Braley-Mullen, H. et al. (2018) Immunohorizons. 2(1): 54–66. PubMed
2. Leonard, WJ. et al. (2002) The EMBO Journal 21: 3051
3. Alt, FW. et al. (1995) Immnnity 3: 521
4. Greene, WC. et al. (1990) J Invest Dermatol. 94: 27S
Flow Cytometry
IHC
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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