Anti-Mouse CD25 – Purified

Pricing & Details

Product No.C1189
Clone
PC61
Protein
CD25
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IL2RA, IDDM10, CD25, IL2R, TCGFR, P55, Tac
Isotype
Rat
IgG1 λ
Applications
B
,
Depletion
,
FA
,
FC
,
IHC FF
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1189-100 µg
100 µg
$38.00
In stock
Max:
Min: 1
Step: 1
C1189-500 µg
500 µg
$93.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this PC61 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this PC61 antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
CODEX®
B
Depletion
IHC (Frozen)
IP
FA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone PC61 recognizes an epitope on mouse CD25.
Antigen Distribution
CD25 is expressed in activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells.
Background
CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25.

Antigen Details

Protein
Ligand/Receptor
IL-2
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Warren J. Leonard et al. (2002) The EMBO Journal 21: 3051 2. FW Alt et al. (1995) Immnnity 3: 521 3. Greene WC et al. (1990) J Invest Dermatol. 94: 27S
Flow Cytometry
IHC
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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