Anti-Mouse CD25 – Purified
Anti-Mouse CD25 – Purified
Product No.: C1189
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Clone PC61 Target CD25 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names IL2RA, IDDM10, CD25, IL2R, TCGFR, P55, Tac Isotype Rat IgG1 λ Applications B , Depletion , FA , FC , IHC FF , IP , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen IL-2-dependent cytolytic mouse T-cell clone B6.1 Product Concentration 0.5 mg/ml Formulation This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This purified antibody is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828354 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this PC61 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this PC61 antibody for use in western blotting is 1-10 μg/ml. Additional Applications Reported In Literature ? CODEX® B Depletion IHC (Frozen) IP FA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone PC61 recognizes an epitope on mouse CD25. Background CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25. Antigen Distribution CD25 is expressed in activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells. Ligand/Receptor IL-2 PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1. Warren J. Leonard et al. (2002) The EMBO Journal 21: 3051
2. FW Alt et al. (1995) Immnnity 3: 521
3. Greene WC et al. (1990) J Invest Dermatol. 94: 27S Technical ProtocolsB Depletion FA  IHC FF   Certificate of Analysis |
Formats Available
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Prod No. | Description |
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C1924 | |
C1189 | |
C1193 | |
C1192 | |
C1191 | |
C1916 | |
C1917 | |
C1918 | |
C1920 | |
C1922 | |
C526 | |
C1194 | |
C1190 | |
C2845 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
