IL-2-dependent cytolytic mouse T-cell clone B6.1
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
Next Day Ambient
Blue Laser (490 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this PC61 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Reported Applications For Relevant Conjugates ?
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Clone PC61 recognizes an epitope on mouse CD25.
CD25 is expressed in activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells.
CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2RA. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25.
NCBI Gene Bank ID
References & Citations
1. Warren J. Leonard et al. (2002) The EMBO Journal 21: 3051
2. FW Alt et al. (1995) Immunity 3: 521
3. Greene WC et al. (1990) J Invest Dermatol. 94: 27S
Products are for research use only. Not for use in diagnostic or therapeutic procedures.