Anti-Mouse CD278 (ICOS) – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.C2258
Clone
7E.17G9
Protein
CD278
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Inducible Costimulator, ICOS
Isotype
Rat
IgG2b κ
Applications
B
,
FA
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2258-1.0 mg
1.0 mg
$105.00
In stock
Max:
Min: 1
Step: 1
C2258-5.0 mg
5.0 mg
$355.00
In stock
Max:
Min: 1
Step: 1
C2258-25 mg
25 mg
$965.00
In stock
Max:
Min: 1
Step: 1
C2258-50 mg
50 mg
$1,595.00
In stock
Max:
Min: 1
Step: 1
C2258-100 mg
100 mg
$2,300.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Mouse ICOS cDNA and ICOS hexahistidine fusion protein
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 7E.17G9 recognizes an epitope on mouse CD278.
Antigen Distribution
ICOS is expressed on activated T cells and APCs including splenic B cells, dendritic cells, and macrophages.
Background
CD278 (ICOS) belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers (47-57kD) and enhances all basic T cell responses to a foreign antigen, specifically proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B cells. ICOS signaling is also an important regulator for T cell homeostasis. ICOS plays a role in humoral immune response and is thought to have involvement in the development of Th2 cells and to play a protective role in inflammatory autoimmune diseases.

Antigen Details

Protein
Ligand/Receptor
B7h/B7RP-1/GL-50
Function
Costimulates T cell activation, proliferation, humoral immune response
PubMed
NCBI Gene Bank ID
Research Area
Costimulatory Molecules
.
Immunology

References & Citations

1. Flavell, R. A. et al. (2001) Nature 409:97 2. Bachmann, M. F. et al. (2000) J. Exp. Med. 192:53 3. Kroczek, R. A. et al. (1999) Nature 397:263
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.