Anti-Mouse CD54 (Clone YN1/1.7.4) – Purified in vivo GOLD™ Functional Grade

The YN1/1.7.4 clone is a monoclonal antibody that targets mouse CD54 (ICAM-1) and has specific functional properties that make it valuable for in vivo mouse studies investigating immune responses and inflammatory processes.

Functional Properties for In Vivo Applications

The YN1/1.7.4 antibody possesses several key characteristics that enable its use in blocking studies. It has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, which are critical integrin receptors involved in leukocyte adhesion. This blocking capability allows researchers to inhibit specific immune cell interactions in living mice.

The antibody can inhibit cell-cell adhesion and interfere with antigen presentation to T cells and leukocyte migration to inflammatory tissues. These functional properties make it particularly useful for studying the role of CD54-mediated interactions in various immune and inflammatory processes.

Target Molecule and Expression

CD54 (ICAM-1) is a 90 kD immunoglobulin superfamily member that is expressed on activated endothelial cells, high endothelial venules, T and B cells, monocytes/macrophages, granulocytes, and dendritic cells. The molecule participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions through binding to LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18).

Potential In Vivo Study Applications

Based on its blocking properties, the YN1/1.7.4 clone would be useful in mouse studies investigating:

• Inflammatory responses where CD54-mediated leukocyte adhesion and migration need to be blocked
• Immune cell trafficking studies examining how CD54 interactions affect cell movement to inflammatory sites
• T cell activation and antigen presentation mechanisms
• Autoimmune and inflammatory diseases where CD54 upregulation plays a pathological role

The antibody's ability to block CD54 binding to its integrin partners makes it a valuable tool for loss-of-function studies in mice, allowing researchers to determine the specific contribution of CD54-mediated adhesion to various immune processes and disease states.

The correct storage temperature for sterile packaged clone YN1/1.7.4 (such as anti-mouse CD54/ICAM-1 antibody) is 2°C to 8°C.

• The antibody solution should be stored undiluted between 2°C and 8°C and protected from prolonged exposure to light.
• Do not freeze the antibody unless specifically stated otherwise by the manufacturer, as freezing can compromise antibody integrity for many conjugated or functional-grade preparations.
• For functional-grade, sterile products, storage at 2°C to 8°C as received is recommended for up to one month; for longer-term storage, you should consult the specific manufacturer’s instructions.
• Most directly labeled or conjugated antibodies, including those using fluorescent dyes like Pacific Blue™, should not be frozen and must be protected from light.

These guidelines are consistent across multiple suppliers and datasheets for clone YN1/1.7.4.

Antibodies and Proteins Commonly Used with YN1/1.7.4 in Research

The YN1/1.7.4 clone is a monoclonal antibody against mouse CD54 (ICAM-1), widely used in immunology and cell biology studies, particularly for detecting, blocking, or functional interrogation of CD54’s roles in cell adhesion and immune interactions.

Key Context of Use

• LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18): CD54’s primary ligands are the integrins LFA-1 and Mac-1, which mediate its role in cell adhesion, leukocyte migration, and immune synapse formation. Antibodies or blocking reagents for LFA-1 and Mac-1 are often used alongside YN1/1.7.4 to dissect these interactions in vitro and in vivo.
• Rhinovirus: CD54 is also the receptor for rhinoviruses, the principal causative agents of the common cold. Studies examining rhinovirus entry or neutralization may use YN1/1.7.4 in combination with viral proteins or antiviral antibodies.
• General Immune Cell Markers: In flow cytometry or histology, YN1/1.7.4 is frequently paired with antibodies against other cell surface markers (e.g., CD3, CD4, CD8, CD19, CD11b, CD11c) to identify specific immune cell populations and their activation states.

Typical Experimental Combinations

Additional Notes

• In Vivo/In Vitro Blocking: YN1/1.7.4 is used both in vitro (e.g., adhesion assays) and in vivo (e.g., inflammation models) to block CD54 function, often in conjunction with reagents targeting LFA-1 or Mac-1 for comprehensive inhibition of leukocyte adhesion.
• Multicolor Flow Cytometry: YN1/1.7.4 is available conjugated to various fluorophores (e.g., PE, PE-Cy5, APC), enabling its use in multicolor panels with other lineage or activation markers.
• Functional Studies: Beyond detection, YN1/1.7.4 is employed in functional studies of antigen presentation, leukocyte migration, and inflammation, where it may be used with cytokines (e.g., TNF-?, IFN-?) that induce CD54 expression.

Conclusion

The YN1/1.7.4 antibody is most commonly used in combination with antibodies against LFA-1 and Mac-1 to study cell adhesion and immune cell interactions, and with rhinovirus or antiviral reagents in virology contexts. It is also routinely paired with general immune cell markers in multicolor flow cytometry to characterize CD54-expressing populations in complex samples. These combinations reflect CD54’s central role in immunity, inflammation, and viral pathogenesis.

Clone YN1/1.7.4 is a rat monoclonal antibody targeting mouse CD54 (ICAM-1), widely used in immunological research to study cell adhesion, inflammatory response, and antigen presentation. Key findings from its citations in scientific literature are:

• Binding and Imaging: Clone YN1/1.7.4 rapidly binds to endothelial cell membranes, a property utilized for fluorescence-guided imaging and fiber-optic micronavigation in ex vivo liver models. When used at doses around 1200 ng, it achieves effective tissue labeling, with fluorescence signal and mean fluorescence intensity (MFI) enhanced when combined with other endothelial antibodies (e.g., clone 390 against CD31). Increasing doses above this threshold may diminish antibody capture efficacy.

• Functional Blockade: This clone is reported to block the interaction of CD54 (ICAM-1) with its integrin ligands LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18), thereby inhibiting cell-cell adhesion. It is used experimentally to impede T cell and leukocyte adhesion and migration during inflammatory responses, and to study the role of ICAM-1 in antigen presentation and leukocyte recruitment.

• Applications and Specificity:

  • Flow cytometry: YN1/1.7.4 is employed for flow cytometric analysis of endothelial and immune cell expression of CD54.
  • Research areas: It is applied in immunology, cell adhesion studies, and research on inflammation, innate immunity, and antigen presentation.
  • It specifically recognizes mouse CD54/ICAM-1 and has been used to study its cellular localization (membrane-associated) and function.

• Biological Importance: The molecule it targets, ICAM-1, is crucial for lymphocyte trafficking, T cell-B cell interactions, and as an entry receptor for certain viruses (e.g., rhinovirus).

• Blocking Migration and Inflammation: Studies using this antibody have shown that blocking CD54 can reduce leukocyte migration to inflamed tissues, supporting its role as a valuable tool for dissecting immune cell trafficking mechanisms.

In summary, literature citing YN1/1.7.4 demonstrates its value in:

• Imaging and quantifying endothelial cell labeling
• Blocking adhesion and migration in immune cell studies
• Dissecting mechanistic roles of ICAM-1 in inflammation

These findings make it a critical reagent in mouse immunology, especially when analyzing endothelial activation and leukocyte trafficking.