Anti-Mouse CD70 (Clone FR70) – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.C2402
Clone
FR70
Protein
CD70
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TNFSF7, KI-24 antigen, CD27 Ligand, CD27L
Isotype
Rat
IgG2b κ
Applications
FA
,
FC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2402-0.5 mg
0.5 mg
$655.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
BALB/c mouse B lymphoma A20.J2
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this FR70 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this FR70 antibody for use in western blotting is 1-10 μg/ml.
Additional Reported Applications For Relevant Conjugates ?
IP
B
IHC (Frozen)
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone FR70 recognizes an epitope on mouse CD70.
Antigen Distribution
CD70 is expressed by highly activated lymphocytes as found in T-cell and B-cell lymphomas.
Background
CD70 is a 30-33 kD type II transmembrane glycoprotein and a member of the TNF superfamily. CD70 is the ligand for CD27; the binding of which delivers costimulatory signals to T cells and NK cells. CD70 is expressed on highly activated lymphocytes characteristic of T- and B-cell lymphomas. Because normal lymphocytes have low CD70 expression, it has been suggested that anti-CD70 antibodies may be a possible treatment for CD70 positive lymphomas. CD70-specific antibodies are currently under investigation for the treatment of hematological malignancies and renal cell carcinoma.

Antigen Details

Protein
Ligand/Receptor
CD27
Function
T-B cell cross-stimulation
PubMed
NCBI Gene Bank ID
Research Area
Costimulatory Molecules
.
Immunology
Flow Cytometry
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.