Anti-Mouse CD71 – Purified

Pricing & Details

Product No.C275
Clone
R17217
Protein
CD71
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Transferrin Receptor
Isotype
Rat
IgG2a
Applications
FA
,
FC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C275-50 µg
50 µg
$60.00
In stock
Max:
Min: 1
Step: 1
C275-500 µg
500 µg
$210.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this R17217 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this R17217 antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
CODEX®
Additional Reported Applications For Relevant Conjugates ?
B
FA
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone R17217 recognizes an epitope on CD71.
Antigen Distribution
CD71 is expressed on most proliferating cells, activated lymphocytes, monocytes, macrophages, erythroid progenitors, and brain endothelium.
Background
CD71 is a 95 kD type II homodimeric transmembrane glycoprotein. The function of CD71 is believed to be primarily nutritional. It plays a role in the control of cellular proliferation through facilitation of iron uptake by way of ferrotransferrin binding and the recycling of apotransferrin to the cell surface. Additionally, transferrin receptor is required for erythropoiesis and proper neurological development, and it has also been suggested that a growth signal might be generated by the transferrin/transferrin receptor interaction. Current genetic analysis indicates that the structural genes for transferrin receptor and for a melanoma-associated antigen (p97), and perhaps transferrin itself, each map to a common chromosome in humans. These proteins exhibit primary sequence homology with transferrin and have the ability to bind ferric iron. Therefore, it is thought that genetic rearrangements in this iron transport region may be associated with malignant transformation. Hence, anti-CD71 mAbs are thought to have therapeutic potential in cases of human leukemia & lymphoma.

Antigen Details

Protein
Ligand/Receptor
Transferrin
PubMed
NCBI Gene Bank ID

References & Citations

1. Lesley, J. et al. (1982) Immunogenetics 15:313
2. Lesley, J. et al. (1984) Mol. Cell. Biol. 4(9):1675
3. Kemp, JD. et al. (1987) J. Immunol. 138:2422
4. Kemp, JD. et al. (1989) Cell Immunol. 122:218
Flow Cytometry
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.