Anti-Mouse GM-CSF – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.G670
Clone
MP1-22E9
Protein
GM-CSF
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CSF-2, MGI-1GM, Pluripoietin-Alpha
Isotype
Rat
IgG2a κ
Applications
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
G670-1.0 mg
1.0 mg
$98.00
In stock
Max:
Min: 1
Step: 1
G670-5.0 mg
5.0 mg
$325.00
In stock
Max:
Min: 1
Step: 1
G670-25 mg
25 mg
$875.00
In stock
Max:
Min: 1
Step: 1
G670-50 mg
50 mg
$1,450.00
In stock
Max:
Min: 1
Step: 1
G670-100 mg
100 mg
$2,125.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Yeast Derived Recombinant Mouse GM-CSF
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Additional Reported Applications For Relevant Conjugates ?
N
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone MP1-22E9 recognizes an epitope on mouse GM-CSF. This antibody shows no cross-reactivity with rhGM-CSF.
Background
Granulocyte-Macrophage Colony Stimulating Factor is a 22 kD, pleiotropic cytokine that is a white blood cell growth factor. It controls the production and function of blood cells by stimulating stem cells to produce granulocytes and monocytes. GM-CSF differs from G-CSF in that it affects more cell types including macrophages and eosinophils. Moreover, GM-CSF is part of the immune/inflammatory cascade, a process crucial for fighting infection. Interestingly, GM-CSF expression may have pathological implications. Autocrine expression of GM-CSF in myeloid leukemia cells is suspected to play a role in neoplasia, the formation of a new and abnormal growth of tissue. Additionally, GM-CSF expression has also been documented in certain solid tumors. There have also been reports of GM-CSF in synovial fluid from patients with arthritis suggesting that GM-CSF may play a role in tissue damage associated with the inflammatory process. Blocking GM-CSF is thought to have therapeutic potential by reducing inflammation. Some drugs are currently being developed to block GM-CSF.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
.
Stem Cell

References & Citations

1. Parker, MW. et al. (2008) Cell 134: 496
2. Whitsett, JA. et al. (2002) Annual Review of Physiology 64: 775
General Western Blot Protocol