Anti-Mouse H-2Kb (MHC Class I) [Clone Y-3] – Purified in vivo GOLD™ Functional Grade
Anti-Mouse H-2Kb (MHC Class I) [Clone Y-3] – Purified in vivo GOLD™ Functional Grade
Product No.: Y100
Clone Y-3 Target MHC Class I Formats AvailableView All Product Type Monoclonal Antibody Alternate Names H-2K, H-2 class I histocompatibility antigen, K-B alpha chain, H-2K(B), H-2K(K), H-2K(Q), H-2K(R), H-2K(S), Beta-2-microglobulin Isotype Mouse IgG2b κ Applications FA , ICC , in vivo , IP , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Con A stimulated spleen cells from BALB.B mice Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737575 Additional Applications Reported In Literature ? FC WB ICC IP FA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone Y-3 recognizes an epitope on mouse MHC class I H-2K haplotypes b, k, q, r, s, but not d. Background H-2K antibody, clone Y-3, recognizes the major histocompatibility complex (MHC) class I H-2K haplotypes b, k, q, r, s, but not d. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. H-2K plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11. Antigen Distribution H-2K is ubiquitously expressed on nucleated cells. Research Area Immunology . Innate Immunity Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone Y-3 is most commonly used in vivo in mice to block MHC class I (specifically H-2K^b^ and related haplotypes) function, thereby inhibiting MHC class I–mediated T cell responses. Key in vivo applications include:
Secondary laboratory applications (in vitro and ex vivo, but sometimes related to in vivo studies) include:
Notes:
There is some confusion in the literature with clone Y-3 versus Y-3P; Y-3P is an anti–MHC class II (I-A) antibody, while Y-3 is specific for MHC class I (H-2K^b^). This answer pertains to clone Y-3 (not Y-3P) and its in vivo uses. Other commonly used antibodies or proteins applied in conjunction with Y-3 (anti-mouse H-2K^b, MHC Class I, Clone Y3) in the literature include actin, GFP, CD markers such as CD31, and various secondary antibodies for detection. These are employed to assess protein loading, label specific cell types, or facilitate visualization and quantification in immunological and cell biology assays. Key details:
Other studies may include antibodies against other MHC class I or II proteins and proteins relevant to the experimental disease model or transgenic strain under investigation. Frequently, multi-antibody panels are built to dissect complex cellular landscapes or monitor immune cell activation and trafficking. In summary, Y-3 is most commonly paired with structural proteins (like actin), lineage or state markers (CD markers, GFP), and secondary detection reagents. This enhances cell-type specificity and methodological rigor across diverse immunological assays. Key Findings from clone Y-3 (Anti-H-2Kb) in Scientific LiteratureSpecificity and Application
Functional Insights
Availability and Validation
Limitations in Literature Coverage
Summary Table
ConclusionClone Y-3 is a well-established tool for identifying and functionally blocking mouse H-2Kb, with recognized specificity and utility across a range of immunological assays. While it is widely referenced and available commercially, detailed mechanistic findings or breakthrough discoveries specifically attributed to clone Y-3 in the scientific literature are scarce in the context of citation summaries; most references are from reagent providers summarizing its general use and validation. Researchers should consult primary literature and experimental methods for in-depth examples of clone Y-3 in action. The dosing regimens for clone Y-3, an antibody targeting mouse MHC Class I (H-2Kb), can vary significantly across different mouse models. While specific dosing protocols for clone Y-3 are not detailed in the current search results, general guidelines for similar antibodies suggest that dosing often depends on several factors:
For antibodies targeting similar immune components, dosages often range from 200 to 500 μg per mouse per injection, with administration every 2 to 3 days being common. However, without specific studies or protocols for clone Y-3, these general guidelines from related antibodies should be considered as a starting point, and actual dosing may need to be optimized based on empirical data from specific mouse models. References & Citations1. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):10618-25. 2. Wieczorek M, et al. (2017) Front Immunol. 8:292. 3. Jones EY. (1997) Curr Opin Immunol. 9(1):75-9. 4. Matsumura M, et al. Science (1992) 257:927–34.10.1126/science.1323878 5. Bouvier M & Wiley DC. (1994) Science. 265:398–402.10.1126/science.8023162 6. Zacharias M & Springer S. (2004) Biophys J. 87:2203–14.10.1529/biophysj.104.044743 7. Cruz FM, et al. (2017) Annu Rev Immunol. 35:149-176. 8. Artyomov MN, et al. (2010) Proc Natl Acad Sci USA. 107(39):16916-16921. 9. Orr MT & Lanier LL. (2010) Cell. 142(6):847-856. 10. Raulet DH. (1994) Adv Immunol. 55:381-421. 11. Salcedo M & Ljunggren HG. (1996) Chem Immunol. 64:44-58 Technical ProtocolsCertificate of Analysis |
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