Anti-Mouse/Human CD44 (Clone IM7) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

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Pricing & Details

Product No.C501
Clone
IM7
Protein
CD44
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Pgp-1, Ly-24, H-CAM
Isotype
Rat
IgG2b
Applications
CODEX®
,
IHC FF
,
IHC FFPE
Prod No.
Size
Price
Avail.
Qty
Add to cart
C501-50 µg
50 µg
$95.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

CODEX® Details

Validation Notes
Clone IM7 has been validated for use on CODEX® using human tonsil formalin-fixed paraffin-embedded (FFPE) and mouse spleen fresh frozen (FF) tissues.
Tissue Screened
Spleen
Tonsil
Tissue Preparation
FF (Fresh Frozen)
FFPE (Formalin-Fixed Paraffin-Embedded)

Antibody and Reporter Details

Reactivity Species
Human
Mouse
Host Species
Rat
Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD44 (Clone IM7) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2893562
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone IM7 recognizes an epitope common to alloantigens and all isoforms of CD44 that is located between amino acids 145 and 186.
Antigen Distribution
CD44 is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells in addition to B-cells, monocytes, macrophages and certain subsets of thymocytes and peripheral T-cells. Mice with the Ly-24.1 allotype have high densities of CD44+ T-cells.
Background
CD44 is an 80-95 kD glycoprotein that plays a role in various cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. CD44 interacts with osteopontin, collagens, and matrix metalloproteinases (MMPs) and is a receptor for hyaluronic acid. Transcripts for this gene go through intricate alternative splicing that result in a variety of functionally distinct isoforms, including those which may be related to tumor metastasis. These splice variants of CD44 function as receptors under hemodynamic flow conditions that are significant to the development of cancer metastasis. Hence, it is thought that anti-CD44 tumor-specific mAbs may have therapeutic potential. This therapeutic potential of anti-CD44 mAbs is evident in some animal experiments demonstrating a reduction in malignant activities of various neoplasms when CD44 was targeted by a combination of mAbs, antisense oligonucleotides, and CD44-soluble proteins. It has been reported that high levels of CD44 on leukemic cells fuel leukemia production. Notably, various cancer studies show conflicting results pertaining to level of CD44 expression and its correlation with disease prognosis. Before anti-CD44 therapy can be applied to human cancers, it is essential to resolve this inconsistency.

Antigen Details

Protein
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
PubMed
NCBI Gene Bank ID

References & Citations

1. Trowbridge, I. et al. (1982) Immunogenetics 15:299 2. Lesley, J. et al. (1988) Cell Immunol. 112:40
IHC
IHC

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.