Partially-purified T cell clone supernatant
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions in accordance to our in-house quality systems SOPBR's.
Storage and Handling
This R-PE conjugated antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
Next Day 2-8°C
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry:This antibody can be used at a concentration of ≤ 0.5 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume). Each lot of this antibody is quality control tested.
Other Applications Reported In Literature ?
Additional reported applications (for the relevant formats) include:
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Clone TRFK5 recognizes mouse/human IL-5.
Mast cells, T cells, eosinophils
IL-5 is a 26 kD TH2 cytokine and a homodimeric glycoprotein that is part of the hematopoietic family. IL-5 stimulates B cell growth as well as increases Ig secretion. It is the main factor that promotes the terminal differentiation of eosinophil progenitors and enhances the effector capacity of mature eosinophils. IL-5 is a major regulator of eosinophil accumulation in tissues. In fact, in Hodgkin lymphoma, the characteristically observed eosinophilia is suspected to result from an increased production of IL-5. IL-5 may also be the cause of several allergic diseases including asthma, where it is thought that eosinophils have a significant role in the disease pathology. There are several mAbs currently in use that target IL-5 for the treatment of severe eosinophilic asthma.
Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
NCBI Gene Bank ID
Products are for research use only. Not for use in diagnostic or therapeutic procedures.