Anti-Mouse ICAM-1 (CD54) (Clone BE29G1) – Purified in vivo PLATINUM™ Functional Grade

The clone BE29G1 is an antibody specific for mouse intercellular adhesion molecule 1 (ICAM-1) and is used in in vivo mouse studies primarily to block or detect ICAM-1 in functional assays and tumor models.

In referenced in vivo studies, BE29G1 is used with BALB/c mice:

• It is typically administered to modulate or block ICAM-1 function in the context of immune responses, notably in tumor settings. For example, in the cited study, BE29G1 was applied to examine its effect on tumor progression and immune interactions in mice injected with tumor cells.
• The antibody may be used to assess ICAM-1’s role in immune cell recruitment or adhesion, as part of experiments evaluating cellular vaccines or antitumor immune responses.

Key usage details:

• Mice are injected with tumor cells (subcutaneously or intraperitoneally), and BE29G1 is administered to manipulate ICAM-1 activity as part of experimental immunotherapy protocols or mechanistic studies.
• BE29G1 facilitates in vivo assessment of how ICAM-1 blockade influences tumor growth, immune cell infiltration, or cytokine production (e.g., IFN-? in ELISPOT assays).

In summary, clone BE29G1 is used in vivo to target ICAM-1 in mice, helping researchers study its immunological functions, frequently within tumor immunology and immunotherapy models by modulating or blocking ICAM-1 interactions.

The correct storage temperature for the sterile packaged clone BE29G1 (Anti-Mouse ICAM-1, CD54, Clone BE29G1) is 2–8°C (refrigerated). Do not freeze this product.

This storage range preserves the stability of the purified antibody, and freezing should be specifically avoided as it may denature or damage the product. Always consult the product datasheet or label for clone-specific instructions, but for BE29G1, refrigeration is the standard requirement.

When BE29G1, an anti-mouse ICAM-1 antibody, is used in research, it is often part of broader studies involving inflammation, lymphocyte differentiation, and activation. While specific studies may not always directly mention other antibodies or proteins used alongside BE29G1, common proteins and antibodies in these contexts include:

1. Adhesion Molecules and Integrins:

   • LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) are frequently studied alongside ICAM-1 due to their interactions. These molecules are crucial in cell adhesion processes, similar to ICAM-1.
   • Other adhesion molecules, such as VCAM-1, can also be involved in experiments exploring complex inflammatory responses.

2. Cytokines and Chemokines:

   • Proteins like TNF-? (Tumor Necrosis Factor-alpha) and IL-1? (Interleukin-1 beta) are commonly used to stimulate or monitor inflammatory responses, often in conjunction with ICAM-1 studies.

3. Secondary Antibodies for Detection:

   • Secondary antibodies conjugated with enzymes like HRP (Horseradish Peroxidase) or AP (Alkaline Phosphatase) are used for detection in assays like ELISA or Western blotting. These secondary antibodies are typically specific to the isotype of the primary antibody, such as anti-Rat IgG2a for BE29G1 .

4. Other Monoclonal Antibodies:

   • Monoclonal antibodies targeting specific cell surface markers, such as CD3, CD4, CD8, or CD19, are used to identify and isolate different lymphocyte subsets during studies involving lymphocyte differentiation and activation.

In general, these proteins and antibodies are used to understand the complex interactions in immune responses, particularly in the context of inflammation and cell adhesion.

Clone BE29G1 is a monoclonal antibody specific for mouse intercellular adhesion molecule 1 (ICAM-1), and its key use in the scientific literature is as a tool for detecting or targeting mouse ICAM-1, particularly in immunological or cancer research contexts.

Key findings from scientific citations involving clone BE29G1 include:

• Specificity: BE29G1 is used to specifically recognize mouse ICAM-1 (CD54), a molecule involved in immune cell adhesion and trafficking.
• Experimental application: It appears in immunological assays, notably in studies examining tumor-immune system interactions. For example, in a cancer immunology paper, BE29G1 was used to detect mouse ICAM-1 in functional assays that looked at antigen presentation and T cell activation in response to tumor cell-derived signals.
• Research context: The antibody aids in evaluating the role of ICAM-1 in processes such as leukocyte adhesion, T cell activation, and the immune response to tumors.

In summary, citations of clone BE29G1 in the literature primarily reflect its role as a specific tool for identifying and studying mouse ICAM-1 functions in immunological experiments and cancer immunology research. No conflicting findings about its characterization or specificity appear in the indexed citations.