Anti-Mouse ICAM-1 (CD54) (Clone BE29G1) – Purified in vivo PLATINUM™ Functional Grade

Home/Bulk In vivo Functional Grade Antibodies, Platinum Purified Bulk Antibodies, Primary Monoclonal Antibodies, Surfacetag Mouse CD Markers/Anti-Mouse ICAM-1 (CD54) (Clone BE29G1) – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C991
Clone
BE29G1
Protein
ICAM-1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
BB2, CD54, P3.58
Isotype
Rat
IgG2a
Applications
B
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C991-1.0 mg
1.0 mg
$198.00
In stock
Max:
Min: 1
Step: 1
C991-5.0 mg
5.0 mg
$398.00
In stock
Max:
Min: 1
Step: 1
C991-25 mg
25 mg
$1,150.00
In stock
Max:
Min: 1
Step: 1
C991-50 mg
50 mg
$1,825.00
In stock
Max:
Min: 1
Step: 1
C991-100 mg
100 mg
$2,500.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse ICAM-1 (>98%)
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
WB
Other Applications Reported In Literature ?
B
IP
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone BE29G1 recognizes mouse ICAM-1.
Antigen Distribution
CD54 is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes.
Background
ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Research Area
Cell Adhesion
.
Cell Biology
.
Neuroscience
.
Neuroscience Cell Markers

References & Citations

1. Li S et al. (2009) Biochem Biophys Res Commun. 381: 459 2. Wolf S et al. (2009) Pharmacol Rep 61: 22 3. Ozcan U et al. (2009) Arch Gynecol Obstet.
Immunoprecipitation Protocol
General Western Blot Protocol
Prod No.
Description
R1364