Anti-Mouse IFNγ (Clone H22) – Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse IFNγ (Clone H22) – Purified in vivo PLATINUM™ Functional Grade

Product No.: I-1190

[product_table name="All Top" skus="I-1190"]

- -
- -
Clone
H22
Target
IFN-γ
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFNy
Isotype
IgG
Applications
ELISA
,
IF
,
in vivo
,
IP
,
N
,
WB

- -
- -
Select Product Size

Data

- -
- -

Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Armenian Hamster
Immunogen
Purified Recombinant Mouse IFN-γ (>98%)
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
WB The suggested concentration for this clone H22 antibody for use in western blotting is 0.5 μg/ml.
Additional Applications Reported In Literature ?
N
ELISA
IF
IP

For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Armenian Hamster Anti-Mouse Interferon Gamma (IFN-γ) (Clone H22) recognizes an epitope on Mouse IFN-γ. This antibody was also pathogen tested and third-party certified by IDEXX BioReseach to meet the lowest mycoplasma specification and free of any viral pathogens of concern.
Background
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.3 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.4 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.5

Antigen Details

Ligand/Receptor
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
NCBI Gene Bank ID
Research Area
Immunology
.
Other Molecules

References & Citations

1.) Schreiber, RD. et al. (2017) Cancer Immunol Res. 5(2):106-117. PubMed
2.) Schreiber, RD. et al. (2015) PLoS One.10(5):e0128636. PubMed
3.) Goeddel, DV. et al. (1982) Nature 298: 859
4.) Wilson, CB. et al. (2007) Adv. Immunol. 96: 41
5.) Hume, DA. et al. (2004) J Leukoc Biol. 75: 163
6.) Karki et al. (2021) Cell. 184:149–168 Journal Link
Indirect Elisa Protocol
IF
in vivo Protocol
Immunoprecipitation Protocol
N
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.