Anti-Mouse IFNγ (Clone H22) – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.I-438
Clone
H22
Protein
Interferon Gamma
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI
Isotype
Armenian Hamster
IgG
Applications
IP
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-438-1.0 mg
1.0 mg
$98.00
In stock
Max:
Min: 1
Step: 1
I-438-5.0 mg
5.0 mg
$325.00
In stock
Max:
Min: 1
Step: 1
I-438-25 mg
25 mg
$875.00
In stock
Max:
Min: 1
Step: 1
I-438-50 mg
50 mg
$1,450.00
In stock
Max:
Min: 1
Step: 1
I-438-100 mg
100 mg
$2,125.00
In stock
Max:
Min: 1
Step: 1

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Armenian Hamster
Immunogen
Purified Recombinant Mouse IFN-γ (>98%)
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: For Western blotting, the suggested use of clone H22 antibody is 0.6 µg/ml., (See Image Above).
Additional Reported Applications For Relevant Conjugates ?
N
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Armenian Hamster Anti-Mouse Interferon-Gamma (IFN-γ) (Clone H22) recognizes an epitope on Mouse IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.4 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.5 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.6

Antigen Details

Ligand/Receptor
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Schreiber, RD. et al. (2017) Cancer Immunol Res. 5(2):106-117. PubMed
2. Diamond, MS. et al. (2017) J Virol. 91(22): e01419-17. PubMed
3. Schreiber, RD. et al. (2015) PLoS One.10(5):e0128636. PubMed
4. Goeddel, DV. et al. (1982) Nature 298: 859
5. Wilson, CB. et al. (2007) Adv. Immunol. 96: 41
6. Hume, DA. et al. (2004) J Leukoc Biol. 75: 163
7.) Winkler, E. et al. (2020) Cell 182(4):901-918.e18 Journal Link
Immunoprecipitation Protocol
General Western Blot Protocol
Prod No.
Description
R1364