Anti-Mouse IFN-γ (Clone XMG1.2) – Purified

Anti-Mouse IFN-γ (Clone XMG1.2) – Purified

Product No.: I-1104

[product_table name="All Top" skus="I-1104"]

- -
- -
Clone
XMG1.2
Target
IFN-γ
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI
Isotype
IgG1
Applications
ELISA Cap
,
ELISPOT
,
ICFC
,
IHC FFPE
,
N

- -
- -
Select Product Size

Data

- -
- -

Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse IFN-γ (>98%)
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Cap XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA with a concentration range of 0.5-2.0 µg/ml as recommended.
WB The suggested concentration for this XMG1.2 antibody for use in western blotting is 1-10 μg/ml.
Additional Applications Reported In Literature ?
ICFC
ELISPOT Cap
ELISPOT Det
N
Additional Reported Applications For Relevant Conjugates ?
IHC FF
WB

For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Interferon Gamma (IFN-γ) (Clone XMG1.2) recognizes an epitope on Mouse IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3

Antigen Details

PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
.
Immunology
.
Neuroinflammation
.
Neuroscience
.
Other Molecules

References & Citations

1. Goeddel DV et al. (1982) Nature 298: 8592. Wilson CB et al. (2007) Adv. Immunol. 96: 413. Hume DA et al. (2004) J Leukoc Biol. 75: 163
Elisa Sandwich Protocol
ELISPOT
ICFC
IHC FFPE
N