Anti-Mouse IFN-γ (Clone XMG1.2) – FITC

Anti-Mouse IFN-γ (Clone XMG1.2) – FITC

Product No.: I-1120

[product_table name="All Top" skus="I-1120"]

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Clone
XMG1.2
Target
IFN-γ
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI
Isotype
IgG1
Applications
ICFC

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse IFN-γ (>98%)
Product Concentration
0.2 mg/ml
Formulation
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This Fluorescein (FITC) conjugate is stable when stored at 2-8°C. Do not freeze.This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Blue Laser (490 nm)
Additional Applications Reported In Literature ?
ICFC The suggested concentration for clone XMG1.2 FITC conjugated antibody for staining cells in Intracellular Immunofluorescent flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Interferon Gamma (IFN-γ) (Clone XMG1.2) recognizes an epitope on Mouse IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3

Antigen Details

PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
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Immunology
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Neuroinflammation
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Neuroscience

References & Citations

1. Goeddel DV et al. (1982) Nature 298: 859
2. Wilson CB et al. (2007) Adv. Immunol. 96: 41
3. Hume DA et al. (2004) J Leukoc Biol. 75: 163
4. Teijaro et al. (2020) bioRxiv 22: 24
5. Feldmann et al. (2021) Microorganisms 9(2): 279