Anti-Mouse IFNγ (Clone XMG1.2) – Purified in vivo GOLD™ Functional Grade
Pricing & Details
Purified Recombinant Mouse IFN-γ (>98%)
≥ 5.0 mg/ml
< 1.0 EU/mg as determined by the LAL method
≥95% monomer by analytical SEC
>95% by SDS Page
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Cap XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA with a concentration range of 0.5-2.0 µg/ml as recommended.
WB The suggested concentration for this XMG1.2 antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
Additional Reported Applications For Relevant Conjugates ?
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rat Anti-Mouse Interferon Gamma (IFN-γ) (Clone XMG1.2) recognizes an epitope on Mouse IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3
NCBI Gene Bank ID
References & Citations
1). Goeddel DV et al. (1982) Nature 298: 859
2.) Wilson CB et al. (2007) Adv. Immunol. 96: 41
3.) Hume DA et al. (2004) J Leukoc Biol. 75: 163
4.) Teijaro et al. (2020) bioRxiv 22: 24
5.) Hawman DW, et al. (2021) Microorganisms 9(2):279 Journal Link