Anti-Mouse IL-17 (Clone 50104)

Anti-Mouse IL-17 (Clone 50104)

Product No.: I-667

[product_table name="All Top" skus="I-667"]

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Clone
50104
Target
IL-17
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CTLA8, IL17A, Interleukin-17
Isotype
IgG2a
Applications
ELISA Indirect
,
N
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WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse IL-17 (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse IL-17 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-533.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Mouse IL-17 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IL-17 (at 10 ng/ml) is 0.05 - 0.15 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Rat Anti-Mouse Interleukin 17 (IL-17) (Clone 50104) recognizes an epitope on Mouse IL-17. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody does not cross-react with rhIL-17, rhIL-17B, rhIL-17C, rmIL-17E, or rmIL-17F.
Background
Interleukin 17 (IL-17) is a T cell cytokine that induces monocyte migration and inflammation.1 IL-17-producing cell could serve as a potential prognostic marker and a novel therapeutic target for hepatocellular carcinoma (HCC).2 IL-17 may be important in recruiting monocytes into the joints of patients with RA, supporting IL-17 as a therapeutic target in RA.1
PubMed

References & Citations

1. Pope, RM. et al. (2009) J Immunol. 182: 3884
2. Zheng, L. et al. (2009) J Hepatol. 50(5):980-989.
Indirect Elisa Protocol
N
General Western Blot Protocol

Certificate of Analysis

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Formats Available

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Prod No.
Description
I-667
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.