Anti-Mouse TNFα – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.T703
Clone
TN3-19.12
Protein
TNF-α
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TNF-alpha, TNFSF2, Cachectin, Differentiation-Inducing Factor (DIF), Necrosin, Cytotoxin
Isotype
Armenian Hamster
IgG
Applications
IP
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
T703-1.0mg
1.0 mg
$198.00
In stock
Max:
Min: 1
Step: 1
T703-5.0mg
5.0 mg
$398.00
In stock
Max:
Min: 1
Step: 1
T703-25mg
25 mg
$1,150.00
In stock
Max:
Min: 1
Step: 1
T703-50mg
50 mg
$1,825.00
In stock
Max:
Min: 1
Step: 1
T703-100mg
100 mg
$2,500.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Armenian Hamster
Immunogen
Purified Recombinant Mouse TNF-α
Product Concentration
≥ 2.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
WB
Other Applications Reported In Literature ?
IP
Additional Reported Applications For Relevant Conjugates ?
N
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone TN3-19.12 recognizes mouse TNFα.
Antigen Distribution
TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, B cells, NK cells, LAK cells.
Background
TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Antigen Details

Protein
Ligand/Receptor
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
PubMed
NCBI Gene Bank ID

References & Citations

1.) El-Harith el-HA et al. (2004) Saudi Med J. 25: 135
2.) Adolf GR et al. (1990) Infec Immun. 58: 3996
3.) Kanneganti et al. (2020) bioRxiv 361048 Journal Link
4.) Fhogartaigh, C.N. et al. (2020) Nat Commun 11, 6385 Journal Link
5.) Karki et al. (2021) Cell. 184:149–168 Journal Link
6.) Karki, R. et al. (2021) Cell 184(1):149–168.e17 Journal Link
Immunoprecipitation Protocol
General Western Blot Protocol