Donkey F(ab’)2 Anti-Mouse IgG (H&L) (Adsorbed Against: Ms., Hu., Rt., Rb., Shp., Hs., GP., CK., Sy. Hms.) – HRP

Home/Secondary Antibodies AffiPure and Streptavidin/F(ab')2 Fragment/Donkey F(ab’)2 Anti-Mouse IgG (H&L) (Adsorbed Against: Ms., Hu., Rt., Rb., Shp., Hs., GP., CK., Sy. Hms.) – HRP

Pricing & Details

Prod. No.M1171
Clone
Product Type
Secondary Antibody
Alternate Names
Isotype
Donkey
Workshops
Size
Price
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0.3 ml
217.00
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Donkey
Immunogen
Purified Mouse IgG (H&L) (>98%)
Product Concentration
0.8 mg/ml
Formulation
This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)
Product Preparation
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Storage and Handling
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. Caution: This horseradish peroxidase conjugate does not contain preservatives. To avoid microbial contamination, store the reconstituted conjugate containing glycerol at -20°C in a manual defrost freezer.
Expiration Date
One year from date of reconstitution
Country of Origin
USA
Shipping
Next Day Ambient
Working Concentration
WB: 1:5,000 - 1:100,000
WB Chemiluminescent: 1:10,000 - 1:200,000
ELISA: 1:5,000 - 1:100,000
IHC: 1:500 - 1:5,000
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Donkey F(ab')2 Anti-Mouse IgG (H&L) recognizes Mouse IgG heavy and light chains. This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein
PubMed
Products are for research use only. Not for use in diagnostic or therapeutic procedures.