Donkey F(ab')2 Anti-Rabbit IgG (H&L) (Adsorbed Against: Ms., Hu., Rt., Bv., Shp., Hs., GP., CK., Sy. Hms.) – Alk Phos

Pricing & Details

  
Product Type
Secondary Antibody
Prod No.
Size
Price
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R1203-0.3 ml
0.3 ml
$205.00
In stock
  

Antibody Details

Reactivity Species
Rabbit
Host Species
Donkey
Immunogen
Purified Rabbit IgG (H&L) (>98%)
Product Concentration
0.6 mg/ml
Formulation
This secondary antibody is lyophilized from 0.01M Tris buffered saline (TBS) pH 8.0 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.
Product Preparation
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Storage and Handling
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Alkaline Phosphatase (Alk Phos) conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.
Expiration Date
One year from date of reconstitution
Working Concentration
WB: 1:5,000 - 1:50,000
ELISA: 1:5,000 - 1:50,000
Applications and Recommended Usage ?
(Quality Tested by Leinco)
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.

IHC (NBF/Par.): This antibody may be used to stain nuclear membranes in cells, tissue preparations or subcellular fractions. The antibody works well with cells and tissue sections fixed with 2% paraformaldehyde and requires no dilution. Tonsil is the recommended positive control tissue.

Flow Cytometry: PN:A106

Western Blotting: To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents such as PN:M114 and a chromogenic substrate such as PN:T343, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions.
Specificity
Donkey F(ab')2 Anti-Rabbit IgG (H&L) recognizes Rabbit IgG heavy and light chains. This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein Family
IgG (H&L)
  
Products are for research use only. Not for use in diagnostic or therapeutic procedures.