Donkey F(ab’)₂ Anti-Rabbit IgG (H&L) (Adsorbed Against: Ms., Hu., Rt., Bv., Shp., Hs., GP., CK., Sy. Hms.) – Biotin

Purified Rabbit IgG (H&L) (>98%)

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Biotin conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.

One year from date of reconstitution

Next Day 2-8°C

WB: 1:20,000 - 1:400,000
ELISA: 1:20,000 - 1:400,000
IHC: 1:500 - 1:5,000
FC: 1:200 - 1:1,000

Donkey F(ab')₂ Anti-Rabbit IgG (H&L) recognizes Rabbit IgG heavy and light chains. This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.