Donkey F(ab’)2 Anti-Rat IgG (H&L) (Adsorbed Against: Hu., Rb., Bv., Shp., Hs., GP., CK., Sy. Hms.) – Biotin

Pricing & Details

  
Product Type
Secondary Antibody
Prod No.
Size
Price
Avail.
Qty
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R967-0.3 ml
0.3 ml
$171.00
In stock
  

Antibody Details

Reactivity Species
Rat
Host Species
Donkey
Immunogen
Purified Rat IgG (H&L) (>98%)
Product Concentration
1.3 mg/ml
Formulation
This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.
Product Preparation
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Storage and Handling
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Biotin conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.
Expiration Date
One year from date of reconstitution
Working Concentration
It is recommended that working dilutions be prepared fresh daily.

WB: 1:20,000 - 1:400,000
ELISA: 1:500 - 1:5,000
IHC: 1:200 - 1:1,000
FC: 1:200 - 1:1,000
IF: 1:200 - 1:1,000

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
Specificity
Donkey F(ab')2 Anti-Rat IgG (H&L) recognizes Rat IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein
IgG (H&L)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.