UltraAvidin™- DyLight® 488

Pricing & Details

  
Prod No.
Size
Price
Avail.
Qty
Add to cart
A425-1.0 mg
1.0 mg
$100.00
In stock
A425-2.0 mg
2.0 mg
$175.00
In stock

Data

  

Antibody Details

Product Details

Host Species
Chicken Egg White
Product Concentration
1.0 mg/ml
Formulation
UltraAvidin™-DyLight® 488 is supplied in 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
Stable when stored at 2 - 8°C. Do not freeze
Country of Origin
USA
See directions on lot specific datasheets, as information may periodically change.
Working Concentration
Western blotting: 1:5,000 - 1:10,000
ELISA: 1:5,000 - 1:20,000
Immunohistochemistry: 1:100 - 1:1,000
Western blotting and ELISA Chemiluminescent: 1:20,000 - 1:100,000

However, each investigator should determine their own optimal working dilution for each specific research application.
Isoelectric Point
pI= 6.3 +/- 0.3
Specific Activity
11-17 µg biotin bound/mg UltraAvidin™
Each investigator should determine their own optimal working dilution for specific applications.

Description

Background
UltraAvidin is Leinco Technologies' trademark for a uniquely modified form of avidin which is isolated from chicken egg whites. With a molecular weight of 60,000 there are four identical subunits each capable of binding one molecule of biotin. UltraAvidin from Leinco Technologies has been de-glycosylated to prevent carbohydrate moieties from adhering to lectin-like receptors on the surface of cells, thus eliminating the possibility of false positives. Unlike native avidin, UltraAvidin has a near neutral pI which prevents electrostatic interactions with negatively charged serum or membrane proteins.

Antigen Details

References & Citations

1. Wilchek, M. et al.(1990) Biochem Biophys Res Commun. 1990 170(3):1236-41.
2. Sullivan, W. et al. (1996) Genetics. 143(4):1629-42.
  
Products are for research use only. Not for use in diagnostic or therapeutic procedures.