Human Placenta Tissue Lysate

Human Placenta Tissue Lysate

Product No.: P165

[product_table name="All Top" skus="P165"]

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Product Type
Tissue Lysate

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Product Size
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Details

Tissue Type
Human Placenta
Diagnosis
Normal
Preparation
Placenta tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Safety Testing
This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.
Host
Human
Concentration
2.0 mg/ml
Formulation
The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol.
Expiration Date
One year from date of receipt
Storage
For short term storage of this tissue lysate, freeze in single use aliquots at -20°C <b>in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.</b> For long term storage freeze working aliquots at -70°C.
Applications and Suggested Working Dilutions
This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.