Human Placenta Tissue Lysate

Human Placenta Tissue Lysate

Product No.: P165

[product_table name="All Top" skus="P165"]

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Product Type
Tissue Lysate

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Details

Tissue Type
Human Placenta
Diagnosis
Normal
Preparation
Placenta tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Safety Testing
This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.
Host
Human
Concentration
2.0 mg/ml
Formulation
The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol.
Expiration Date
One year from date of receipt
Storage
For short term storage of this tissue lysate, freeze in single use aliquots at -20°C <b>in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.</b> For long term storage freeze working aliquots at -70°C.
Applications and Suggested Working Dilutions
This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.