Recombinant SARS-CoV-2, Trimeric Spike (S) Protein

Pricing & Details

  
Alternate Names
Spike Protein, S Protein, Trimeric Spike Protein, Trimer
Expression Host
HEK-293
Reactivity Species
SARS-CoV-2
Virus
Prod No.
Size
Price
Avail.
Qty
Add to cart
S848-100µg
100 µg
$700.00
In stock
S848-500µg
500 µg
$2,900.00
In stock
S848-1.0mg
1.0 mg
$4,900.00
In stock
  

Background

SARS-CoV-2 is an RNA virus that has a trimeric spike (S) protein on its viral envelope, similar to other enveloped RNA viruses like RSV and Influenza. The trimeric Spike Protein consists of the S1 and S2 domains. The S1 domain contains the receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2) receptors on target cells. SARS-CoV-2 is closely related to the SARS virus, which was first identified in 2002-2003¹. In-depth analysis has identified the SARS-CoV-2 Trimeric as being essential for ACE2 binding². Both SARS-CoV and SARS-CoV-2 utilize the ACE2 cell receptor to gain entry into cells, with SARS-CoV-2 binding with higher affinity³. Vaccine and therapeutic development are targeting the S protein⁴.

Protein Details

Format
Purified No Carrier Protein
Purity
>95% by SDS Page
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Expression Host
HEK-293
Protein Accession No.
QHD43416.1
Amino Acid Sequence
QCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKR FDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVY YHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHT PINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTF LLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFN ATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQ IAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQA GSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVN FNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQ VAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICAS YQTQTNSPGSASSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMY ICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPD PSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSA LLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTA SALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVT QQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKN FTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDP LQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQ
State of Matter
Sterile Liquid
Predicted Molecular Mass
The predicted molecular mass is ~132 kDa for monomer and ~396 kDa for trimer
Formulation
This recombinant protein is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
Storage and Stability
This recombinant protein may be stored as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Shipping
Next Day
NCBI Gene Bank

References & Citations

1. Quinlan et al. bioRxiv: 2020.
2. Lan et al. Nature: 2020.
3. Wrapp et al. Science: 2020.
4. Tai et al. Nature: 2020.
Elisa Sandwich Protocol
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
  
Products are for research use only. Not for use in diagnostic or therapeutic procedures.