UltraAvidin™- DyLight® 650

UltraAvidin™- DyLight® 650

Product No.: A428

[product_table name="All Top" skus="A428"]

- -
- -
Product Type
Avidin - Biotin

- -
- -
Select Product Size
- -
- -


UltraAvidin is Leinco Technologies' trademark for a uniquely modified form of avidin which is isolated from chicken egg whites. With a molecular weight of 60,000 there are four identical subunits each capable of binding one molecule of biotin. UltraAvidin from Leinco Technologies has been de-glycosylated to prevent carbohydrate moieties from adhering to lectin-like receptors on the surface of cells, thus eliminating the possibility of false positives. Unlike native avidin, UltraAvidin has a near neutral pI which prevents electrostatic interactions with negatively charged serum or membrane proteins.

Protein Details

DyLight® 650
Product Concentration
0.2 mg/ml
This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Working Concentration
Western blotting: 1:5,000 - 1:10,000
ELISA: 1:5,000 - 1:20,000
Immunohistochemistry: 1:100 - 1:1,000
Western blotting and ELISA Chemiluminescent: 1:20,000 - 1:100,000

However, each investigator should determine their own optimal working dilution for each specific research application.
Storage and Stability
Stable when stored at 2 - 8°C. Do not freeze
Country of Origin
Next Day 2-8°C

References & Citations

1. Wilchek, M. et al. (1990) Biochem Biophys Res Commun. 170(3):1236-41.
2. Sullivan, W. et al. (1996) Genetics. 143(4):1629-42.
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.