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Anti-Human CD3 (CD3) Purified

Monoclonal Antibody


Prod. No.:C105
Clone:UCHT-1
Isotype:Mouse IgG1
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Anti-Human CD3 recognizes a (Mr 22-28 kDa) T-cell surface glycoprotein. The epitope recognized by the CD3 antibody is expressed on a constant region of the epsilon chain of the CD3 antigen/T-cell receptor complex (TCR). This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL cells.

Background:
Anti-CD3 may be used for enumerating immunocompetent T-lymphocytes in peripheral blood. Clone UCHT-1 is also useful in histology for localization of T-lymphocytes in tissue and may be used to enrich T-cells by cell sorting. This T-cell marker has also been used to determine T-versus B-cell lymphomas and leukemias.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This antibody is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC, IP, WB
Flow Cytometry: This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. Each investigator should determine their own optimal working dilution for specific applications.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Leukocyte Typing VI (1996) Oxford University Press
2. van Dongen, J. J. M. et al. (1988) Blood 71:603
3. Meuer, S. C. et al. (1983) Nature 303:808
4. Beverly, P.C.L. et al. (1981) Eur. J. Immunol. 11:329
5. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
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