Anti-Glutathione-S-Transferase (GST) Antibody, HRP conjugate (18844P)
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Antibody DetailsProduct DetailsHost Species Rabbit Immunogen Glutathione-S-Transferase (GST) from Schistosoma japonicum. Product Concentration 1.0 mg/ml Formulation Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400 State of Matter Liquid Product Preparation Purified by affinity on GST Storage and Handling This antibody is stable for at least one (1) year at 2-8°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at a dilution of 1:1,000- 1:30,000.
Immunocytochemistry: use at a dilution of 1:200-1:500 ELISA: use at a dilution of 1:10,000- 1:100,000 These are recommended dilutions. Endusers should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to Glutathione-S-Transferase (GST). Background A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids which, compared to tags such as the myc-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. Many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification. Antigen DetailsFunction Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.; GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut. UniProt.org Research Area Epitope Tags References & CitationsTechnical Protocols |
