Anti-Human CD14 – Biotin

Anti-Human CD14 – Biotin

Product No.: C154

[product_table name="All Top" skus="C154"]

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Clone
UCHM-1
Target
CD14
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
P35
Isotype
IgG2a
Applications
FC

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this UCHM-1 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone UCHM-1 recognizes an epitope on human CD14.
Background
CD14 is a 53-55 kD GPI-anchored single chain glycoprotein that plays a role in the clearance of gram-negative pathogens by interacting with other proteins, including TLR-4 and MD-2, to mediate the innate immune response to bacterial lipopolysaccharide (LPS). As a high-affinity receptor for LPS, it is involved in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.
Antigen Distribution
CD14 is expressed on monocytes, macrophages, dendritic cells, and granulocytes.

Antigen Details

Ligand/Receptor
LPS
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
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Immunology
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Neuroinflammation
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Neuroscience

References & Citations

1. Freyssinet, JM et al. (1997) J Clin Invest. 99(7): 1546–1554. PubMed
2. Ulrich, Costabel et al. (1995) CHEST 108: 1614
3. Ulevitch, RJ et al. (1993) Immunobiology 187: 227
4. Schumann, RR et al. (1994) Medical Microbiol Immunol. 183: 279
5. Verhasselt, V et al. (1997) J Immunol. 158: 2919
6. Kirkland, TN et al. (2000) J Biol Chem. 275: 3144
Flow Cytometry

Certificate of Analysis

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.