Anti-Human CD14 – Purified in vivo GOLD™ Functional Grade

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Next Day 2-8°C

Clone UCHM-1 recognizes an epitope on human CD14.

CD14 is a 53-55 kD GPI-anchored single chain glycoprotein that plays a role in the clearance of gram-negative pathogens by interacting with other proteins, including TLR-4 and MD-2, to mediate the innate immune response to bacterial lipopolysaccharide (LPS). As a high-affinity receptor for LPS, it is involved in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.

CD14 is expressed on monocytes, macrophages, dendritic cells, and granulocytes.

LPS

1. Ulrich Costabel et al. (1995) CHEST 108: 1614 2. Ulevitch RJ et al. (1993) Immunobiology 187: 227 3. Schumann RR et al. (1994) Medical Microbiol Immunol. 183: 279 4. Verhasselt V et al. (1997) J Immunol. 158: 2919 5. Kirkland TN et al. (2000) J Biol Chem. 275: 3144