Anti-Human CD14 – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.C370
Clone
UCHM-1
Protein
CD14
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
P35
Isotype
Mouse
IgG2a
Applications
FC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C370-1.0 mg
1.0 mg
$198.00
In stock
Max:
Min: 1
Step: 1
C370-5.0 mg
5.0 mg
$398.00
In stock
Max:
Min: 1
Step: 1
C370-25 mg
25 mg
$1,150.00
In stock
Max:
Min: 1
Step: 1
C370-50 mg
50 mg
$1,625.00
In stock
Max:
Min: 1
Step: 1
C370-100 mg
100 mg
$2,220.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this UCHM-1 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this UCHM-1 antibody for use in western blotting is 1-10 μg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone UCHM-1 recognizes an epitope on human CD14.
Antigen Distribution
CD14 is expressed on monocytes, macrophages, dendritic cells, and granulocytes.
Background
CD14 is a 53-55 kD GPI-anchored single chain glycoprotein that plays a role in the clearance of gram-negative pathogens by interacting with other proteins, including TLR-4 and MD-2, to mediate the innate immune response to bacterial lipopolysaccharide (LPS). As a high-affinity receptor for LPS, it is involved in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.

Antigen Details

Protein
Ligand/Receptor
LPS
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
.
Immunology
.
Neuroinflammation
.
Neuroscience

References & Citations

1. Ulrich Costabel et al. (1995) CHEST 108: 1614 2. Ulevitch RJ et al. (1993) Immunobiology 187: 227 3. Schumann RR et al. (1994) Medical Microbiol Immunol. 183: 279 4. Verhasselt V et al. (1997) J Immunol. 158: 2919 5. Kirkland TN et al. (2000) J Biol Chem. 275: 3144
Flow Cytometry
General Western Blot Protocol