Anti-Human CD8 [Clone UCHT-4] — Purified in vivo GOLD™ Functional Grade
Anti-Human CD8 [Clone UCHT-4] — Purified in vivo GOLD™ Functional Grade
Product No.: C366
Clone UCHT-4 Target CD8 Formats AvailableView All Product Type Monoclonal Antibody Isotype Mouse IgG2a Applications FA , FC , in vivo , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Thymocytes and Sézary T cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829678 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this UCHT-4 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this UCHT-4 antibody for use in western blotting is 1-10 μg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone UCHT-4 recognizes human CD8. Background CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes. Antigen Distribution CD8 is expressed on blood lymphocytes, a subset of NK cells, and thymocytes. Persons with HIV exhibit increased levels of CD8+ lymphocytes. Ligand/Receptor MHC Class I molecules PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone UCHT-4 is a mouse monoclonal antibody specific to the human CD8 antigen, and its common in vivo applications in mice are primarily in studies involving humanized or xenograft mouse models containing human immune cells. Key applications include:
Limitations:
The antibody is not used for immune cell manipulation in wild-type mice or for any mouse-specific cell depletions, for which anti-mouse CD8 clones (e.g., YTS 169, 53-6.7) are required. Commonly used antibodies or proteins paired with UCHT-4 (a monoclonal antibody against human CD8) in the literature are other monoclonal antibodies or detection reagents that target related markers, especially in immunology and flow cytometry experiments. In experimental contexts, the following are frequently used together with UCHT-4:
In flow cytometry or cell sorting, these combinations enable multi-parametric analysis:
These antibodies are often conjugated to different fluorochromes to distinguish them during multiplexed assays. The exact panel depends on the experimental question, but CD3 and CD4 are the most common co-used antibodies with UCHT-4 in immunological studies. Clone UCHT-4 is a monoclonal antibody widely cited in scientific literature for its specificity to human CD8, predominantly marking cytotoxic T cells and some regulatory T cell subsets. The principal findings from publications citing UCHT-4 include:
Key publications that cite UCHT-4 have reinforced its status as a benchmark reagent for defining CD8+ lymphocytes in human samples, supporting its widespread adoption in research and diagnostics. No significant alternative or conflicting uses of clone UCHT-4 are described in the indexed search results. Its main scientific impact derives from its reliable specificity and utility in immunological studies targeting human CD8+ T cells. Dosing regimens for clone UCHT-4 (an anti-human CD8 monoclonal antibody) in mouse models are not explicitly detailed in the provided search results, and published protocols are limited compared to more common depleting clones such as GK1.5 (anti-CD4) and 2.43 (anti-CD8α) used in mice. However, general principles can be inferred:
Summary Table:
No direct, controlled, comparative studies of clone UCHT-4 dosing regimens across different mouse models are present in the search results. Most laboratories optimize based on depletion efficiency, host strain, and antibody pharmacokinetics in a given model, generally starting from regimens published for analogous monoclonal antibodies. If you have a specific mouse model or experimental context (e.g., depletion of human CD8+ T cells in NSG mice engrafted with human PBMCs), this would enable a more tailored dosing guidance. References & Citations1. Parnes, J. R. et al. (1989) Adv. Immunol. 44:265
2. Reinherz, E. L. et al. (1980) J. Immunol. 124:1301
3. Fischer, A. et al. (1983) Immunology 48:177
4. Merkenschlanger, M. et al. (1988) Eur. J. Immunol. 18:1653
5. Leukocyte Typing: 3rd Workshop: Code No. 567; 4th Workshop: Code No. N31 Technical ProtocolsCertificate of Analysis |
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