Anti-Human IFN-γ

Product No.: I-646

[product_table name="All Top" skus="I-646"]

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Interferon Gamma
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI

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Antibody Details

Product Details

Reactive Species
Host Species
Purified Recombinant Human IFN-γ (Accession # AAP20098.1)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ICFC Intracellular Staining by Flow Cytometry suitable for use at concentration of 0.25 µg/106 cells
Additional Applications Reported In Literature ?
NThis antibody is useful for neutralization of Human IFN-γ bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IFN-γ (at 50 ng/ml) is 0.02 - 0.06 µg/ml.
ICC Immunocytochemistry suitable for use at concentration of 8-25 µg/mL.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


Mouse Anti-Human Interferon Gamma (IFN-γ) (Clone 25718) recognizes an epitope on Human IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3

Antigen Details

Research Area
Other Molecules

References & Citations

1. Goeddel, DV. et al. (1982) Nature 298: 859
2. Wilson, CB. et al. (2007) Adv. Immunol. 96: 41
3. Hume, DA. et al. (2004) J Leukoc Biol. 75: 163

Formats Available

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Prod No.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.