Anti-Mouse IFN-γ

Product No.: I-669

[product_table name="All Top" skus="I-669"]

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Clone
37895
Target
Interferon Gamma
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Immune Interferon, Type II Interferon, T Cell Interferon, MAF
Isotype
IgG2a
Applications
CyTOF®
,
FC
,
N
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
E. coli-Derived Recombinant Mouse IFN-γ
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IFN-γ. A suggested method would be to stain cells expressing IFN-γ with 0.25 µg per 1 - 2.5 x 106 cells in a total staining volume of ≤200 µl followed by PN:R485.
WB To detect Mouse IFN-γ in western blotting, this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IFN-γ is 100 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Additional Applications Reported In Literature ?
N This antibody is useful for neutralization of Mouse IFN-γ bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IFN-γ (at 2.5 ng/ml) is 0.075 - 0.3 µg/ml.
CyTOF® Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Interferon Gamma (IFN-γ) (Clone 37895) recognizes an epitope on Mouse IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody does not cross-react with rhIFN-γ, rrIFN-γ, rpIFN-γ, rrmIFN-γ, rfeIFN-γ or rcaIFN-γ.
Background
Mouse IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulates the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-gamma species-specific and is biologically active only in human and primate cells.

Antigen Details

Research Area
Other Molecules
CyTOF®
Flow Cytometry
N
General Western Blot Protocol

Formats Available

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Prod No.
Description
I-669
Products are for research use only. Not for use in diagnostic or therapeutic procedures.