Anti-Human IL-17
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human Il-17A (>98%) Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830553 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human IL-17 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-17 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-198. Flow Cytometry: Suitable for use at concentration of 2.5 µg/106 cells Additional Applications Reported In Literature ? Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 2-15 µg/ml. Immunoprecipitation: Suitable for use at concentration of 3 µg/100 µg cell lysate. Immunocytochemistry: Suitable for use at concentration of 5-15 µg/mL. Neutralization: Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 0.02-0.12 µg/mL in the presence of 15 ng/mL Recombinant Human IL‑17. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Human Interleukin-17 (IL-17) recognizes Human IL-17. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Interleukin 17 (IL-17) is a T cell cytokine that induces monocyte migration and inflammation.1 IL-17-producing cell could serve as a potential prognostic marker and a novel therapeutic target for hepatocellular carcinoma (HCC).2 IL-17 may be important in recruiting monocytes into the joints of patients with RA, supporting IL-17 as a therapeutic target in RA.1 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Pope, RM. et al. (2009) J Immunol. 182: 3884 2. Zheng, L. et al. (2009) J Hepatol.50(5):980-9. Technical ProtocolsCyTOF®  ICC IHC FF  N  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
