Anti-Human MIP-3α (Clone 67310)

Pricing & Details

Product No.M1093
Clone
67310
Protein
MIP-3 alpha
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CKb4, Liver Activation Regulated Chemokine (LARC), MIP3A, SCYA20, ST38, Exodus-1, CCL20
Isotype
Mouse
IgG1
Applications
ELISA Cap
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
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M1093-25 µg
25 µg
$205.00
In stock
Max:
Min: 1
Step: 1
M1093-100 µg
100 µg
$389.00
In stock
Max:
Min: 1
Step: 1
M1093-500 µg
500 µg
$920.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human MIP-3α (Accession # P78556.1)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 µg/ml. A suitable detection antibody is PN:M197 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:M167.
Western Blotting: To detect Human MIP-3α this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M167.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human MIP-3α bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human MIP-3α (at 10 ng/ml) is 1 - 5 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Macrophage Inflammatory Protein-3 Alpha (MIP-3α) (Clone 67310) recognizes an epitope on Human MIP-3α. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
MIP-3α is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils.1 MIP-3α is specifically expressed by the follicle-associated epithelia (FAE) covering intestinal Peyer's patches (PPs) and is the only known chemokine ligand for the chemokine receptor CCR6.2 MIP3-α is produced by intestinal epithelial cells (including cells associated with mucosal lymphoid tissues), by keratinocytes in inflamed skin, and by cells in liver and lung.3,4 It plays an important role in the inflammatory response of blood monocytes and tissue macrophages.

Antigen Details

References & Citations

1. Nomiyama, H. et al. (1997) J. Biol. Chem. 272: 5846
2. Kucharzik, T. et al. (2005) J. Pathol. 166: 1647
3. Cook, DN. et al. (2000) Immunity 12: 495
4. Farber, JM. et al. (2002) J. Immunol. 168: 4871
Elisa Sandwich Protocol
General Western Blot Protocol

Formats Available

Prod No.
Description
M1093
Products are for research use only. Not for use in diagnostic or therapeutic procedures.