Anti-Rat MIP-3α

Pricing & Details

Product No.C1468
Protein
MIP-3 alpha
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
CCL20, CKb4, Liver Activation Regulated Chemokine (LARC), MIP3A, SCYA20, ST38, Exodus-1
Applications
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1468-25 µg
25 µg
$185.00
In stock
Max:
Min: 1
Step: 1
C1468-100 µg
100 µg
$410.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Rat
Host Species
Goat
Immunogen
E. coli-Derived Recombinant Rat CCL20
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Rat CCL20 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Rat CCL20 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
Neutralization: Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND50) is typically 30-75 µg/mL in the presence of 5 µg/mL Recombinant Rat CCL20/MIP‑3 alpha.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Rat Macrophage Inflammatory Protein 3 Alpha (MIP-3α) recognizes Rat MIP-3α. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Macrophage Inflammatory Protein-3 Alpha (MIP3A) also known as CCL20 or liver activation regulated chemokine (LARC) is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils.1 MIP3A is specifically expressed by the follicle-associated epithelia (FAE) covering intestinal Peyer's patches (PPs) and is the only known chemokine ligand for the chemokine receptor CCR6.2 MIP3-alpha is produced by intestinal epithelial cells (including cells associated with mucosal lymphoid tissues), by keratinocytes in inflamed skin, and by cells in liver and lung.3,4 It plays an important role in the inflammatory response of blood monocytes and tissue macrophages.

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Nomiyama, H. et al. (1997) J. Biol. Chem. 272: 5846
2. Kucharzik, T. et al. (2005) J. Pathol. 166: 1647
3. Cook, DN. et al. (2000) Immunity 12: 495
4. Farber, JM. et al. (2002) J. Immunol. 168: 4871
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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