Anti-Mouse MIP-3α (Clone 114906)

Pricing & Details

Product No.M1285
Clone
114906
Protein
MIP-3 alpha
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CKb4, Liver Activation Regulated Chemokine (LARC), MIP3A, SCYA20, ST38, Exodus-1
Isotype
Rat
IgG1
Applications
ELISA Indirect
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
M1285-25 µg
25 µg
$185.00
In stock
Max:
Min: 1
Step: 1
M1285-100 µg
100 µg
$389.00
In stock
Max:
Min: 1
Step: 1
M1285-500 µg
500 µg
$920.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
E. coli-Derived Recombiant Mouse MIP-3α
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse MIP-3α this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse MIP-3α is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Other Applications Reported In Literature ?
Neutralization: Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 ng/mL Recombinant Mouse CCL20/MIP‑3 alpha.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Macrophage Inflammatory Protein 3 Alpha (MIP-3α) (Clone 114906) recognizes an epitope on Mouse MIP-3α. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Macrophage Inflammatory Protein-3 Alpha (MIP3A) also known as CCL20 or liver activation regulated chemokine (LARC) is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils.1 MIP3A is specifically expressed by the follicle-associated epithelia (FAE) covering intestinal Peyer's patches (PPs) and is the only known chemokine ligand for the chemokine receptor CCR6.2 MIP3-alpha is produced by intestinal epithelial cells (including cells associated with mucosal lymphoid tissues), by keratinocytes in inflamed skin, and by cells in liver and lung.3,4 It plays an important role in the inflammatory response of blood monocytes and tissue macrophages.

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Nomiyama, H. et al. (1997) J. Biol. Chem. 272: 5846
2. Kucharzik, T. et al. (2005) J. Pathol. 166: 1647
3. Cook, DN. et al. (2000) Immunity 12: 495
4. Farber, JM. et al. (2002) J. Immunol. 168: 4871
Indirect Elisa Protocol
General Western Blot Protocol

Formats Available

Prod No.
Description
M1285
Products are for research use only. Not for use in diagnostic or therapeutic procedures.