Anti-Mouse MIP-3α
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Goat Immunogen E. coli-Derived Recombinant Mouse MIP-3α Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831292 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse MIP-3α this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse MIP-3α is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse MIP-3α bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse MIP-3α (at 20 ng/ml) is 0.5 - 2 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Mouse Macrophage Inflammatory Protein 3 Alpha (MIP-3α) recognizes Mouse MIP-3α. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Macrophage Inflammatory Protein-3 Alpha (MIP3A) also known as CCL20 or liver activation regulated chemokine (LARC) is a small cytokine belonging to the CC chemokine family. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils.1 MIP3A is specifically expressed by the follicle-associated epithelia (FAE) covering intestinal Peyer's patches (PPs) and is the only known chemokine ligand for the chemokine receptor CCR6.2 MIP3-alpha is produced by intestinal epithelial cells (including cells associated with mucosal lymphoid tissues), by keratinocytes in inflamed skin, and by cells in liver and lung.3,4 It plays an important role in the inflammatory response of blood monocytes and tissue macrophages. Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Nomiyama, H. et al. (1997) J. Biol. Chem. 272: 5846 2. Kucharzik, T. et al. (2005) J. Pathol. 166: 1647 3. Cook, DN. et al. (2000) Immunity 12: 495 4. Farber, JM. et al. (2002) J. Immunol. 168: 4871 Technical ProtocolsN  |
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