Anti-Human VEGF (Clone 26503)

Product No.: V103


Clone 26503 Target VEGF Formats AvailableView All Product Type Monoclonal Antibody Alternate Names VEGFA, MGC70609, VPF, VAS, Folliculostellate Cell-Derived Growth Factor, Glioma-Derived Endothelial Cell Mitogen, Vascular Endothelial Growth Factor Isotype IgG2b Applications ELISA Cap , IHC FFPE , N , WB


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human VEGF (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2832130 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.2-0.8 µg/ml. A suitable detection antibody is PN:V105 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:V101. Western Blotting: To detect Human VEGF this monoclonal antibody can be used at a concentration of 0.1 -0.2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human VEGF is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:V101. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. Neutralization: This antibody is useful for neutralization of Human VEGF bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human VEGF (at 10 ng/ml) is 0.04 - 0.08 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Mouse Anti-Human Vascular Endothelial Growth Factor (VEGF) (Clone 26503) recognizes an epitope on Human VEGF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.^1,2 VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.^3,4,5,6 PubMed VEGF References & Citations 1. Folkman, J. et al. (2008) FASEB J. 22: 3728 2. Goodsell, DS. et al. (2002) The Oncologist 7: 569 3. Mugishima, H. et al. (2006) J Atheroscler Thromb. 13: 130 4. Claesson-Welsh, L. et al. (2006) Exp Cell Res. 312: 549 5. Claesson-Welsh, L. et al. (1999) Trends Biochem Sci. 28: 488 6. Ellis, LM. et al. (2005) J Clin Oncol. 23: 1011 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols IHC FFPE N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human VEGF (>98%)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2832130

Applications and Recommended Usage?
Quality Tested by Leinco

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.2-0.8 µg/ml. A suitable detection antibody is PN:V105 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:V101.
Western Blotting: To detect Human VEGF this monoclonal antibody can be used at a concentration of 0.1 -0.2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human VEGF is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:V101.

Additional Applications Reported In Literature ?

IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human VEGF bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human VEGF (at 10 ng/ml) is 0.04 - 0.08 µg/ml.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Vascular Endothelial Growth Factor (VEGF) (Clone 26503) recognizes an epitope on Human VEGF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.^1,2 VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.^3,4,5,6

PubMed

VEGF

References & Citations

1. Folkman, J. et al. (2008) FASEB J. 22: 3728
2. Goodsell, DS. et al. (2002) The Oncologist 7: 569
3. Mugishima, H. et al. (2006) J Atheroscler Thromb. 13: 130
4. Claesson-Welsh, L. et al. (2006) Exp Cell Res. 312: 549
5. Claesson-Welsh, L. et al. (1999) Trends Biochem Sci. 28: 488
6. Ellis, LM. et al. (2005) J Clin Oncol. 23: 1011

View product citations for antibody PRODUCT_CODE on CiteAb

IHC FFPE

Certificate of Analysis

Request COA

Prod No.	Description
V103	Anti-Human VEGF (Clone 26503)
V142	Anti-Rat VEGF (Clone 123704)
V104	Anti-Mouse VEGF
V149	Anti-Rat VEGF
V105	Anti-Human VEGF – Biotin
V106	Anti-Mouse VEGF – Biotin
V160	Anti-Rat VEGF – Biotin

Formats Available

Prod No.	Description
V103	Anti-Human VEGF (Clone 26503)

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Anti-Human VEGF (Clone 26503)