Anti-Human VEGF – Biotin
Pricing & Details
Purified Recombinant Human VEGF (Accession # AAV38412)
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human VEGF this biotin conjugate can be used at a concentration of 0.1-0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human VEGF is 5 - 20 ng/lane under reducing or non-reducing conditions respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:V101.
ELISA Detection: Suitable for use at concentration of 0.1-0.4 µg/mL
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat Anti-Human Vascular Endothelial Growth Factor (VEGF) recognizes Human VEGF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine1 is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.2 VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.3,4,5,6
NCBI Gene Bank ID
References & Citations
1. Folkman, J. et al. (2008) FASEB J. 22:3728
2. Goodsell, DS. et al. (2002) The Oncologist 7:569
3. Mugishima, H. et al. (2006) J Atheroscler Thromb. 13:130
4. Claesson-Welsh, L. et al. (2006) Exp Cell Res. 312:549
5. Claesson-Welsh, L. et al. (1999) Trends Biochem Sci. 28:488
6. Ellis, LM. et al. (2005) J Clin Oncol. 23:1011
Products are for research use only. Not for use in diagnostic or therapeutic procedures.