Anti-Mouse VEGF

Pricing & Details

Product No.V104
Protein
VEGF
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
Vascular Endothelial Growth Factor, VEGFA, MGC70609, VPF, VAS, Folliculostellate Cell-Derived Growth Factor, Glioma-Derived Endothelial Cell Mitogen
Applications
ELISA Cap
,
IHC FF
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
V104-25 µg
25 µg
$205.00
In stock
Max:
Min: 1
Step: 1
V104-100 µg
100 µg
$445.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Goat
Immunogen
Purified Recombinant Mouse VEGF (>98%)
Endotoxin Level
<0.04 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: Suitable for use at concentration of 0.1 µg/mL.
ELISA Capture: Suitable for use at concentration of 0.2-0.8 µg/mL
Other Applications Reported In Literature ?
Immunohistochemistry: Suitable for use at concentration of 5-15 µg/mL
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Mouse Vascular Endothelial Growth Factor (VEGF) recognizes Mouse VEGF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine1 is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.2 VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.3,4,5,6

Antigen Details

Protein
PubMed
NCBI Gene Bank ID

References & Citations

1. Folkman, J. et al. (2008) FASEB J. 22:3728
2. Goodsell, DS. et al. (2002) The Oncologist 7:569
3. Mugishima, H. et al. (2006) J Atheroscler Thromb. 13:130
4. Claesson-Welsh, L. et al. (2006) Exp Cell Res. 312:549
5. Claesson-Welsh, L. et al. (1999) Trends Biochem Sci. 28:488
6. Ellis, LM. et al. (2005) J Clin Oncol. 23:1011
Elisa Sandwich Protocol
IHC
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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