Anti-Rat VEGF (Clone 123704)
Anti-Rat VEGF (Clone 123704)
Product No.: V142
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Clone 123704 Target VEGF Formats AvailableView All Product Type Monoclonal Antibody Alternate Names VEGFA, MGC70609, VPF, VAS, Folliculostellate Cell-Derived Growth Factor, Glioma-Derived Endothelial Cell Mitogen, Vascular Endothelial Growth Factor Isotype IgG2b Applications ELISA Cap , WB |
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Mouse Immunogen Purified Recombinant Rat VEGF (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2832146 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat VEGF this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Rat VEGF is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:V114. ELISA Capture: Suitable for use at concentration of 2-8 µg/mL. Use in combination with: Detection Reagent: Rat VEGF Biotinylated Antibody. Standard: Recombinant Rat VEGF 164 Protein. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Rat Vascular Endothelial Growth Factor (VEGF) (Clone 123704) recognizes an epitope on Rat VEGF. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine1 is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.2 VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.3,4,5,6 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Folkman, J. et al. (2008) FASEB J. 22: 3728 2. Goodsell, DS. et al. (2002) The Oncologist 7: 569 3. Mugishima, H. et al. (2006) J Atheroscler Thromb. 13: 130 4. Claesson-Welsh, L. et al. (2006) Exp Cell Res. 312: 549 5. Claesson-Welsh, L. et al. (1999) Trends Biochem Sci. 28: 488 6. Ellis, LM. et al. (2005) J Clin Oncol. 23: 1011 Technical Protocols  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
