Anti-Rat VEGF – Biotin
Anti-Rat VEGF – Biotin
Product No.: V160
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Target VEGF Product Type Polyclonal Antibody Alternate Names Vascular Endothelial Growth Factor, VEGFA, MGC70609, VPF, VAS, Folliculostellate Cell-Derived Growth Factor, Glioma-Derived Endothelial Cell Mitogen Applications ELISA Det , WB |
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Antibody DetailsProduct DetailsReactive Species Rat Host Species Goat Immunogen Purified Recombinant Rat VEGF (Accession # AAL07526) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2832164 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Rat VEGF this biotin conjugate can be used at a concentration of .01 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:V114. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:V142 as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Rat VEGF. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Rat Vascular Endothelial Growth Factor (VEGF) recognizes Rat VEGF. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Vascular endothelial growth factor (VEGF), a potent proangiogenic cytokine1 is the key signal used by oxygen-hungry cells to promote growth of blood vessels. It binds to specialized receptors on the surfaces of endothelial cells and directs them to build new vessels.2 VEGF are crucial regulators of vascular development during embryogenesis (vasculogenesis) and blood-vessel formation in the adult (angiogenesis). Abnormal VEGF function is associated with inflammatory diseases including atherosclerosis, and hyperthyroidism.3,4,5,6 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Folkman, J. et al. (2008) FASEB J. 22: 3728 2. Goodsell, DS. et al. (2002) The Oncologist 7: 569 3. Mugishima, H. et al. (2006) J Atheroscler Thromb. 13: 130 4. Claesson-Welsh, L. et al. (2006) Exp Cell Res. 312: 549 5. Claesson-Welsh, L. et al. (1999) Trends Biochem Sci. 28: 488 6. Ellis, LM. et al. (2005) J Clin Oncol. 23: 1011 Technical ProtocolsELISA Det  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
