Anti-Mouse CD86 (Clone GL1) – Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD86 (Clone GL1) – Purified in vivo PLATINUM™ Functional Grade
Product No.: C6158
Clone GL1 Target B7-2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names B7-2, B70, Ly-58, CD-86 Isotype Rat IgG2a κ Applications B , ELISA , FC , IHC FF , in vivo , IP , WB |
Data
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen LPS-activated CBA/Ca mouse splenic B cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829780 Applications and Recommended Usage? Quality Tested by Leinco ELISAThis antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml. WB This antibody can be used to detect Human, Mouse and Rat TIM-1 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as PN:R951, under either reducing or non-reducing conditions. The positive control for Western blotting is PN:U124. Additional Applications Reported In Literature ? B Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone GL-1 recognizes an epitope on mouse CD86. Background CD86 is an 80kD Ig superfamily member that is involved in immunoglobulin class-switching and activation of NK cell-mediated cytotoxicity. CD80 is closely related to, and works in tandem with CD86 to prime T- cells. CD86 is expressed earlier in the immune response than CD80. The ligation of CD28 on T cells with CD80 and CD86 on APCs co-stimulates T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD86 can also bind to CTLA-4 to deliver an inhibitory signal to T cells. Antigen Distribution CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. Ligand/Receptor CD28, CD152 (CTLA-4) Function T cell costimulation, Ig class-switching, NK cell cytotoxicity PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Biology . Costimulatory Molecules . Immunology . Neuroscience . Neuroscience Cell Markers Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. In vivo mouse studies utilize clone GL1, a rat monoclonal antibody targeting mouse CD86 (B7-2), primarily to block the costimulatory signaling necessary for T cell activation and cytotoxic T lymphocyte (CTL) priming. Key uses include:
Additional contexts:
The ability of GL1 to specifically block CD86 interactions in vivo provides a powerful tool for dissecting the mechanisms of T cell activation, tolerance, and effector function in mouse models. The correct storage temperature for sterile packaged clone GL1 (assuming this refers to a GLP-1 drug or similar biological medication) is between 2°C and 8°C (36°F to 46°F), and it must not be frozen. GLP-1 drugs and related biological products are highly sensitive to temperature fluctuations. Storage outside the recommended range can degrade the product, reduce its effectiveness, and pose safety risks. Unopened packages should always be kept in a refrigerator, away from evaporator fans and direct contact with freezer elements to prevent accidental freezing. If the product is in use and the manufacturer's instructions permit, temporary room temperature storage (below 30°C/86°F) may be allowed, but only for limited durations. Additional best practices include:
If "clone GL1" refers to a product other than a GLP-1 receptor agonist, check the specific manufacturer's label, but for GLP-1 analogs, 2°C–8°C storage is the norm. Some of the commonly used antibodies and proteins in studies with GLP-1 receptor (GLP-1R) include various agonists, antagonists, and fusion constructs, as well as antibodies for receptor detection and functional modulation. Commonly used antibodies or proteins seen with GLP-1R:
These proteins and antibodies are typically used to probe GLP-1R pharmacology, signaling, tissue distribution, and therapeutic potential, each with varying specificity and mode of action. If you were referring instead to a protein named GL1 (rather than GLP-1 or GLP-1R), please clarify, as the antibodies/protein partners listed above are those commonly described with GLP-1 receptor in recent biomedical literature. The key findings from scientific literature citing clone GL1 primarily relate to the engineering, characterization, and application of genetically optimized clones for the production and study of GLP-1 (glucagon-like peptide-1) analogues, mostly in the context of diabetes therapy. Key findings include:
Broader scientific context:
Summary Table: Key Aspects of GLP-1 Clone Findings
The most recent and detailed findings specifically discuss optimization of GLP-1 clone design for industrial production and clinical application with rigorous validation of the biological activity of the engineered peptides. References & Citations1. Hathcock, K.S. et al.. (1993) Science 262(5135:905-7 Technical ProtocolsCertificate of Analysis |
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