Anti-Mouse CD122 (IL-2Rβ) – Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse CD122 (IL-2Rβ) – Purified in vivo PLATINUM™ Functional Grade

Product No.: C6325

Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IL-2Rβ, Interleukin 2 receptor β chain, IL-2/15Rb
IgG2b κ
in vivo

Product Size

Antibody Details

Product Details

Reactivity Species
Host Species
Rat T-cell line expressing Mouse IL-2Rβ
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
≥98% monomer by analytical SEC
>95% by SDS Page
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this TM-β1 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this TM-β1 antibody for use in western blotting is 1-10 μg/ml.
Additional Applications Reported In Literature ?
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


Clone TM-β1 recognizes an epitope on mouse CD122.
Antigen Distribution
CD122 is expressed on NK cells and at lower levels by T lymphocytes, B lymphocytes, monocytes, and macrophages.
CD122 is a 70-75 kD IL-2 receptor β chain that is a type I membrane protein. CD122 is involved in T cell-mediated immune responses and its activation increases proliferation of CD8+ effector T cells. It exists in three forms with varying degrees of binding affinity with IL-2. The low affinity form is a monomer of the α subunit and has no involvement in signal transduction. The intermediate affinity form is a γ/β heterodimer and the high affinity form is an α/β/γ heterotrimer. The intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. This protein also interacts with the IL-15 receptor.

Antigen Details

IL-2, IL-15
Critical component of IL-2 and IL-15 signaling
NCBI Gene Bank ID
Research Area

References & Citations

1. Burchill, MA. et al. (2007) J. Immunol. 178:280
2. Friedmann, MC. et al. (1996) Proc. Natl. Acad. Sci. (USA) 93:2077
3. Leonard, WJ. et al. (1987) Science 238:75
Flow Cytometry
In vivo
Immunoprecipitation Protocol
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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