Anti-Mouse CD127 (IL-7Rα) [Clone A7R34] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD127 (IL-7Rα) [Clone A7R34] — Purified in vivo GOLD™ Functional Grade
Product No.: C2223
Clone A7R34 Target CD127 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names IL-7Rα, Interleukin-7 receptor alpha, IL-7Rα chain Isotype Rat IgG2a κ Applications B , CyTOF® , FA , FC , IF Staining , IHC FF , in vivo , IP , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen IL-7Ra-IgG1 fusion protein Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737461 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this A7R34 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? B CyTOF® FA IF Staining IHC (Frozen) IP WB Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone A7R34 recognizes an epitope on mouse CD127. Background CD127 (IL7R-α) is a 60-90 kD type I cytokine receptor involved in the regulation of lymphopoiesis. The active receptor is an α/γ chain heterodimer. The common γ chain, which also associates with the IL-2 receptor, serves mainly to activate signal transduction via the IL7R complex. CD127 also interacts with the Thymic Stromal Lymphopoietin Receptor (TSLPR). CD127 determines signaling events via its association with cytoplasmic signaling molecules and has been reported to be a useful marker for identifying memory and effector T cells. Antigen Distribution IL-7Rα is expressed on bone marrow lymphoid precursors, pro-B cells, monocytes/macrophages, NK-cells, naïve T cells, memory CD4+ and CD8+ T cells, intestinal colorectal and renal carcinoma cell lines, and keratinocytes. Ligand/Receptor IL-7 Function T cell and immature B cell proliferation and development PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone A7R34 is an anti-mouse CD127 (IL-7R?) monoclonal antibody that is widely used in in vivo mouse studies to explore the biological roles of CD127, a component critical for lymphocyte development and homeostasis. Its primary applications in in vivo studies include:
A7R34 has helped define the biological importance of CD127 in murine models by enabling both loss-of-function (blocking) and cell-tracking (phenotyping) approaches in a wide variety of immunological contexts. The correct storage temperature for the sterile packaged clone A7R34 (anti-mouse CD127/IL-7R? antibody) is between 2°C and 8°C, and it should be stored undiluted and protected from prolonged exposure to light; do not freeze. For most ready-to-use antibody formulations, this temperature range (standard refrigeration) provides adequate preservation for weeks to months as specified by the manufacturer. If the antibody contains no preservatives (such as in ultra-purified or LEAF preparations), ensure aseptic handling and strictly adhere to the manufacturer's stated shelf life at 2–8°C. If longer-term storage is necessary, consult the specific manufacturer's datasheet to confirm if freezing at -20°C is permitted. However, standard BioLegend and other commercial preparations of clone A7R34 should not be frozen. The A7R34 antibody, which targets mouse IL-7R? (CD127), is most commonly used in combination with antibodies or proteins that help define lymphocyte populations, analyze receptor signaling pathways, and study immune cell development in the literature. Commonly used companion antibodies or proteins with A7R34 include:
Applications most often involve flow cytometry, so the above antibodies/proteins are chosen based on panel design for identifying and characterizing T cell, B cell, or progenitor subsets in mouse models. In summary, A7R34 is most commonly used with antibodies targeting CD132, TSLPR, CD4, CD8, B220/CD19, NK1.1, ?? TCR, and various activation/memory markers, depending on the research focus. The choice is dictated by the immunological population or process under investigation. Clone A7R34 is a well-characterized rat monoclonal antibody that specifically binds to mouse CD127 (Interleukin-7 Receptor alpha chain, IL-7R?) and is widely used to block IL-7 binding and analyze CD127 biology in immunology research. Key findings from scientific literature using citations of clone A7R34 can be summarized as follows:
In summary:
If you require a more detailed breakdown of PubMed-cited studies with experimental outcomes, please clarify further. References & Citations1. Fry TJ et al. (2005) J Immunol. 174: 6571
2. Murre C et al. (2002) Embo J. 21: 103 Technical ProtocolsCertificate of Analysis |
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