Anti-Mouse CD127 (IL-7Rα) [Clone A7R34] — Purified in vivo GOLD™ Functional Grade

Anti-Mouse CD127 (IL-7Rα) [Clone A7R34] — Purified in vivo GOLD™ Functional Grade

Product No.: C2223

[product_table name="All Top" skus="C2223"]

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Clone
A7R34
Target
CD127
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IL-7Rα, Interleukin-7 receptor alpha, IL-7Rα chain
Isotype
Rat IgG2a κ
Applications
B
,
CyTOF®
,
FA
,
FC
,
IF Staining
,
IHC FF
,
in vivo
,
IP
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Recommended Isotype Controls
Recommended Dilution Buffer
Immunogen
IL-7Ra-IgG1 fusion protein
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this A7R34 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
B
CyTOF®
FA
IF Staining
IHC (Frozen)
IP
WB
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone A7R34 recognizes an epitope on mouse CD127.
Background
CD127 (IL7R-α) is a 60-90 kD type I cytokine receptor involved in the regulation of lymphopoiesis. The active receptor is an α/γ chain heterodimer. The common γ chain, which also associates with the IL-2 receptor, serves mainly to activate signal transduction via the IL7R complex. CD127 also interacts with the Thymic Stromal Lymphopoietin Receptor (TSLPR). CD127 determines signaling events via its association with cytoplasmic signaling molecules and has been reported to be a useful marker for identifying memory and effector T cells.
Antigen Distribution
IL-7Rα is expressed on bone marrow lymphoid precursors, pro-B cells, monocytes/macrophages, NK-cells, naïve T cells, memory CD4+ and CD8+ T cells, intestinal colorectal and renal carcinoma cell lines, and keratinocytes.
Ligand/Receptor
IL-7
Function
T cell and immature B cell proliferation and development
PubMed
NCBI Gene Bank ID
Research Area
Immunology

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

Clone A7R34 is an anti-mouse CD127 (IL-7R?) monoclonal antibody that is widely used in in vivo mouse studies to explore the biological roles of CD127, a component critical for lymphocyte development and homeostasis. Its primary applications in in vivo studies include:

  • Blocking IL-7 signaling: A7R34 blocks IL-7 binding to CD127, thereby inhibiting IL-7-mediated signaling pathways in mice. This allows researchers to assess the role of IL-7/IL-7R interactions in immune system development, T and B cell biology, and immune homeostasis.
  • Functional studies: By administering A7R34 in vivo, researchers can deplete or modulate the activity of CD127+ cells, or block downstream signaling, to study the effects on immune responses, such as T cell survival, differentiation, and memory formation.
  • Phenotyping via flow cytometry: Beyond blocking experiments, A7R34 is also used to label and analyze CD127 expression on mouse leukocyte subsets by flow cytometry, often as part of multiparametric immune profiling in studies of normal and pathological immune function.

A7R34 has helped define the biological importance of CD127 in murine models by enabling both loss-of-function (blocking) and cell-tracking (phenotyping) approaches in a wide variety of immunological contexts.

The correct storage temperature for the sterile packaged clone A7R34 (anti-mouse CD127/IL-7R? antibody) is between 2°C and 8°C, and it should be stored undiluted and protected from prolonged exposure to light; do not freeze.

For most ready-to-use antibody formulations, this temperature range (standard refrigeration) provides adequate preservation for weeks to months as specified by the manufacturer. If the antibody contains no preservatives (such as in ultra-purified or LEAF™ preparations), ensure aseptic handling and strictly adhere to the manufacturer's stated shelf life at 2–8°C.

If longer-term storage is necessary, consult the specific manufacturer's datasheet to confirm if freezing at -20°C is permitted. However, standard BioLegend and other commercial preparations of clone A7R34 should not be frozen.

The A7R34 antibody, which targets mouse IL-7R? (CD127), is most commonly used in combination with antibodies or proteins that help define lymphocyte populations, analyze receptor signaling pathways, and study immune cell development in the literature.

Commonly used companion antibodies or proteins with A7R34 include:

  • CD132 (Common ? chain): Forms the high-affinity receptor complex with CD127 and is important for IL-7 signaling, so antibodies against CD132 are often used alongside A7R34 to study receptor composition and function.
  • TSLPR (Thymic Stromal Lymphopoietin Receptor): CD127 can also pair with TSLPR to form the receptor for TSLP; so, TSLPR-specific antibodies are used to differentiate responses to IL-7 versus TSLP.
  • Markers for lymphocyte lineage and maturation:
    • CD4 and CD8: To analyze thymocyte and T cell subsets, antibodies against CD4 and CD8 are frequently included.
    • B cell markers (e.g., B220, CD19): B cell development is affected by IL-7R? signaling, so B cell markers are common in multi-color panels.
    • NK cell markers (such as NK1.1): For distinguishing thymic NK cells expressing CD127.
    • ?? TCR: Used to identify ?? T cells that also express CD127.
  • Activation/Memory markers:
    • CD44, CD62L, and others: Since CD127 expression is modulated during T cell activation and memory formation, these markers are frequently analyzed with A7R34.
  • Isotype controls: Rat IgG2a ? controls are recommended for setting proper baselines in immunoassays using A7R34 to ensure specificity.

Applications most often involve flow cytometry, so the above antibodies/proteins are chosen based on panel design for identifying and characterizing T cell, B cell, or progenitor subsets in mouse models.

In summary, A7R34 is most commonly used with antibodies targeting CD132, TSLPR, CD4, CD8, B220/CD19, NK1.1, ?? TCR, and various activation/memory markers, depending on the research focus. The choice is dictated by the immunological population or process under investigation.

Clone A7R34 is a well-characterized rat monoclonal antibody that specifically binds to mouse CD127 (Interleukin-7 Receptor alpha chain, IL-7R?) and is widely used to block IL-7 binding and analyze CD127 biology in immunology research.

Key findings from scientific literature using citations of clone A7R34 can be summarized as follows:

  • Specificity and Blocking Capability: A7R34 binds specifically to mouse IL-7R? (CD127) and is frequently used in flow cytometry to detect CD127 on various lymphoid cells. It is notable for its ability to completely block the binding of clone S18006K (another anti-IL-7R? antibody) but does not block SB/199. Importantly, it blocks IL-7 binding to CD127, thus inhibiting IL-7-mediated signaling.
  • Functional Implications: Through blocking IL-7 binding, A7R34 has been instrumental for dissecting the biological roles of IL-7R signaling, including regulation of T and B cell development and homeostasis. CD127 is crucial in lymphocyte lineage development, and its absence leads to severe impairment of both B and T cell maturation.
  • Expression Patterns: A7R34 has helped reveal that CD127 is highly expressed on naive and memory T cells, early B and T cell progenitors, and other lymphoid populations. CD127 expression is dynamically regulated—downregulated upon activation and re-expressed during memory T cell differentiation.
  • Autophagy and T Cell Activation: Recent findings indicate that CD127 (IL-7R?) is degraded by autophagy in activated CD4+ T cells. The use of A7R34 contributed to identifying IL-7R? as an autophagy target, linking autophagic regulation to T cell activation and memory formation. Notably, IL-7R? accumulates in autophagy-deficient T cells, a phenomenon that has clarified distinct pathways for receptor regulation and turnover.
  • Other Applications: A7R34 is also used to study dendritic cell biology, where IL-7R? signaling can modulate CD4+ T cell homeostatic proliferation, with implications for immune responses in lymphopenic settings.

In summary:

  • A7R34 is a tool antibody that blocks IL-7 binding, enabling study of CD127 roles in lymphocyte biology and immune regulation.
  • It has provided insights into the regulators of T and B cell development, memory T cell formation, and the interplay between autophagy and cytokine receptor signaling in immune cells.

If you require a more detailed breakdown of PubMed-cited studies with experimental outcomes, please clarify further.

References & Citations

1. Fry TJ et al. (2005) J Immunol. 174: 6571 2. Murre C et al. (2002) Embo J. 21: 103
B
CyTOF®
FA
Flow Cytometry
IF Staining
IHC FF
in vivo Protocol
Immunoprecipitation Protocol
General Western Blot Protocol

Certificate of Analysis

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Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.