Anti-Mouse CD28

Product No.: C1392

[product_table name="All Top" skus="C1392"]

- -
- -
Target
CD28
Product Type
Polyclonal Antibody
Alternate Names
MGC138290, Tp44
Applications
WB

- -
- -
Product Size
- -
- -

Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
NS0 - Derived Mouse CD28 Extracellular Domain (Accession # P31041)
Endotoxin Level
<0.01 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse CD28 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CD28 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:C1341.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse CD28 recognizes Mouse CD28. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Antigen Distribution
CD28 is expressed on thymocytes, CD4+, CD8+ peripheral T cells, NK cells.
Background
CD28 is a 44 kD glycoprotein and a member of the Ig superfamily. In its capacity as a costimulatory receptor, CD28 produces co-stimulatory signals necessary for T cell activation and survival. CD28 is the only B7 receptor constitutively expressed on naive T cells. Without having this CD28/B7 interaction, T cells will fail to respond to their specific antigen when coming in contact with an MHC/antigen complex and thus, are said to be anergic.

Antigen Details

PubMed
NCBI Gene Bank ID
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.